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PCR Enzyme

  • TransFast<sup>®</sup> Taq DNA Polymerase
  • TransFast<sup>®</sup> Taq DNA Polymerase
  • TransFast<sup>®</sup> Taq DNA Polymerase
  • TransFast<sup>®</sup> Taq DNA Polymerase
TransFast<sup>®</sup> Taq DNA PolymeraseTransFast<sup>®</sup> Taq DNA PolymeraseTransFast<sup>®</sup> Taq DNA PolymeraseTransFast<sup>®</sup> Taq DNA Polymerase

TransFast® Taq DNA Polymerase


  • Product Introduction
  • Manual Download
  • References


Description

TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’exonuclease activity.

Highlights

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• High throughput PCR
• Colony PCR

Unit Definition

One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

Quality Control

TransFast® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransFast® Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers.


Storage

at -20 ℃ for two years

Kit Contents

Component

AP101-01/11

AP101-02/12

TransFast® Taq DNA Polymerase

500 units

6×500 units

10× TransFast® Taq Buffer

1.2ml x 1

1.2ml x 6

2.5mM dNTPs

- / 800 μl x 1

- / 800 μl x 6

6×DNA Loading Buffer

1ml x 1

1ml x 6

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