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PCR SuperMix

  • 2×EasyTaq<sup>®</sup> PCR SuperMix
  • 2×EasyTaq<sup>®</sup> PCR SuperMix
  • 2×EasyTaq<sup>®</sup> PCR SuperMix
  • 2×EasyTaq<sup>®</sup> PCR SuperMix
  • 2×EasyTaq<sup>®</sup> PCR SuperMix
  • 2×EasyTaq<sup>®</sup> PCR SuperMix
2×EasyTaq<sup>®</sup> PCR SuperMix2×EasyTaq<sup>®</sup> PCR SuperMix2×EasyTaq<sup>®</sup> PCR SuperMix2×EasyTaq<sup>®</sup> PCR SuperMix2×EasyTaq<sup>®</sup> PCR SuperMix2×EasyTaq<sup>®</sup> PCR SuperMix

2×EasyTaq® PCR SuperMix


  • Product Introduction
  • Manual Download
  • References

Description

EasyTaq® PCR SuperMix is a ready-to-use mixture of EasyTaq® DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2×concentration and used at 1× concentration by adding template, primers and H2O. PCR products are not suitable for PAGE.

• Extension rate is about 1-2 kb/min.

• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors.

• Amplification of genomic DNA fragment up to 4 kb.

Application

Routine PCR

Storage

at -20°C for two years


Kit Contents


Component

AS111-01/11

AS111-02/12

AS111-03/13

AS111-14

2×EasyTaq® PCR SuperMix(-dye)/(+dye)

1 ml

5×1 ml

15×1 ml

6×80 ml (+dye)

ddH2O

1 ml

5 ml

3×5 ml

96×5 ml

Reaction System

Component

Volume

Final Concentration

Template

Variable

As required

Forward Primer (10 μM)

1 μl

0.2 μM

Reverse Primer (10 μM)

1 μl

0.2 μM

2×EasyTaq® PCR SuperMix

25 μl

ddH2O

Variable

-

Total volume

50 μl

-

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