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RT-PCR

  • EasyScript<sup>®</sup> Reverse Transcriptase
  • EasyScript<sup>®</sup> Reverse Transcriptase
EasyScript<sup>®</sup> Reverse TranscriptaseEasyScript<sup>®</sup> Reverse Transcriptase

EasyScript® Reverse Transcriptase


  • Product Introduction
  • Manual Download
  • References


Description

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.

Applications

• First-strand cDNA synthesis
• Multiple copy gene detection

Unit Definition

One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

5×ES RT Buffer

375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

Storage

at -20℃ for two year

Kit Contents



Component

AE101-02

AE101-03

EasyScript® RT

10000 U

5×10000 U

5×ES RT Buffer

200 μl

5×200 μl

Anchored Oligo(dT)18 Primer (0.5 µg/µl)

50 μl

250 μl

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