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  • Product Name: Rinbonuclease Inhibitor
  • Subtitle:
  • Catalog Number: AI101
  • Storage: at -20℃ for two years
  • 币种:  
    • Specification: 2000 units 5×2000 units
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

Ribonuclease Inhibitor is a recombinant protein purified from E. coli strain carrying human placenta ribonuclease inhibitor gene. Ribonuclease Inhibitor specifically inhibits RNase A, RNase B, and RNase C. It is not effective against RNase 1, RNase T1, S1 nuclease, RNase H and aspergillus-originated RNase. It has no inhibition effect on DNA Polymerase, AMV, M-MLV, SP6, T7 and T3 RNA Polymerases.

 

Storage Buffers

50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10 mM DTT, 100 mM NaCl, 50% glycerol

 

Unit Definition

One unit is defined as the amount of enzyme required to inhibit 5 ng RNase A by 50%.

 

Applications

In vitro inhibition of ribonuclease, cDNA synthesis and in vitro transcription and translation.

 

 

Contents& storage

 

1. Reaction Components

2. Incubate
• For anchored oligo(dT)18 primer or GSP, incubate at 42℃ for 30 minutes.
• For random primer, incubate at 25℃ for 10 minutes, then at 42℃ for 30 minutes.
3. Incubate at 85℃ for 5 seconds to inactivate EasyScript®/TransScript® RT.

 

Citations & references

 

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