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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRqPCR and qRT-PCR / TransScript® II Green One-Step qRT-PCR SuperMix
  • Product Name: TransScript® II Green One-Step qRT-PCR SuperMix
  • Subtitle: SuperMix with necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene
  • Catalog Number: AQ311
  • Storage: at -20℃ for one year
  • 币种:  
    • Specification: 100 rxns×20 µlSystems 400 rxns×20 µlSystems
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

TransScript® II Green One-Step qRT-PCR SuperMix combines the first-strand cDNA synthesis and qPCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene specific primers can be used for this kit. TransScript® II Green One-Step qRT-PCR SuperMix contains all the necessary reagents for cDNA synthesis and qPCR except total RNA/mRNA template and gene specific primers.

 

Highlights

• 5 minutes cDNA synthesis
• cDNA synthesis and qPCR are performed in a single tube using gene specific primers with total RNA or mRNA as templates.
• Passive reference dyes are provided for different qPCR instruments.

 

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• Viral RNA and trace RNA detection

 

Passive Reference Dye Selection

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

Kit Contetns

 

Citations & references

 

Literature Journal IF Author Date Link
Transcriptome-wide analysis of TDP-43 binding small RNAs identifies miR-NID1, a novel miRNA that represses NRXN1 expression Genomics 3.01 Fan Z, et al. 2013 Jul  http://www.ncbi.nlm.nih.gov/pubmed/23827811
A differential sequencing-based analysis of the C. elegans noncoding transcriptome RNA 5.088 Xiao T, et al. 2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22345127
Regulatory elements of Caenorhabditis elegans ribosomal protein genes BMC Genomics 4.397 Sleumer, et al. 2012 http://www.biomedcentral.com/1471-2164/13/433?utm_source=dlvr.it&utm_medium=twitter
Functional Characterization of Long Noncoding RNA Lnc_bc060912 in Human Lung Carcinoma Cells Biochemistry  3.015 Huaxia Luo, et al. 2015 Apr http://pubs.acs.org/doi/abs/10.1021/acs.biochem.5b00259
The Loss of MiR-139-5p Promotes Colitis-Associated Tumorigenesis by Mediating PI3K/AKT/Wnt Signaling The International Journal of Biochemistry & Cell Biology 4.046 Rurong Mao, et al.  2015 Oct http://www.sciencedirect.com/science/article/pii/S1357272515300297
Identification of Intermediate-Size Non-Coding RNAs Involved in the UV-Induced DNA Damage Response in C. elegans PLoS One 3.23 Aqian Li, et al. 2012 Nov http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0048066
Comparative Transcriptome Analysis Reveals Different Molecular Mechanisms of Bacillus coagulans 2-6 Response to Sodium Lactate and Calcium Lactate during Lactic Acid Production PLoS One 3.23 Qin J, et al. 2012 Aug  http://www.ncbi.nlm.nih.gov/pubmed/22928635

 

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