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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
  • Product Name: TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix
  • Subtitle:
  • Catalog Number: AT311
  • Storage: at -20℃ for two years
  • 币种:  
    • Specification: 50 rxns×20 µl Systems 100 rxns×20 µlSystems
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Overview

 

Description

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds.

 

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.

 

Applications

Multiple copy and low copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
IFN-α as an Adjuvant for Adenovirus-Vectored FMDV Subunit Vaccine through Improving the Generation of T Follicular Helper Cells PLoS One 3.73 Su C, et al. 2013 Jun  http://www.ncbi.nlm.nih.gov/pubmed/23823532
Natural variation in PTB1 regulates rice seed setting rate by controlling pollen tube growth Nature communication 10.02 Shuangcheng Li,  Wenbo Li,et al. 2013 Nov http://www.nature.com/ncomms/2013/131118/ncomms3793/full/ncomms3793.html
?WT.ec_id=NCOMMS-20131120
Modulation of HBV replication by microRNA-15b through targeting hepatocyte nuclear factor 1α Nucleic Acids Research 8.28 Xiaopeng Dai, et al. 2014 Apr http://nar.oxfordjournals.org/content/early/2014/04/04/nar.gku260.short
Regulation of Tissue LC-PUFA Contents, D6 Fatty Acyl Desaturase (FADS2) Gene Expression and the
Methylation of the Putative FADS2 Gene Promoter by Different Dietary Fatty Acid Profiles in Japanese Seabass (Lateolabrax japonicus)
PLOS ONE 3.23 Houguo Xu, Xiaojing Dong,et al. 2014 Jan http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3909213/
Cytokinin pathway mediates APETALA1function in the establishment of determinate floral meristem in Arabidopsis.  PNAS 9.737 Yingying Han, et al.     2014 May http://www.pnas.org/content/111/18/6840.short
Process of aerenchyma formation and reactive oxygen species induced by waterlogging in wheat seminal roots Planta 3.347 Xu Q T, et al. 2013 Nov http://www.ncbi.nlm.nih.gov/pubmed/23975011
Natural variation in PTB1 regulates rice seed setting
rate by controlling pollen tube growth
Nature communication 10.02 Shuangcheng Li, et al.  2013 Nov http://www.nature.com/ncomms/2013/131118/ncomms3793/full/ncomms3793.html
?WT.ec_id=NCOMMS-20131120
Chrysin promotes osteogenic differentiation via ERK/MAPK activation Protein and Cell 3.22 Zeng W, et al.  2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23744338
Feed intake, growth performance and cholesterol metabolism in juvenile turbot (Scophthalmus maximus L.) fed defatted fish meal diets with graded levels of cholesterol Aquaculture 2.009 Tengfei Zhu, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S0044848614001306
Amino acid substitutions of GLY98, LEU245 and GLU543 in COI1 distinctively affect jasmonate-regulated male fertility in Arabidopsis Science China Life Sciences 1.688 Huang Huang, et al. 2014 Jan http://link.springer.com/article/10.1007/s11427-013-4590-1
Protective effect of polysaccharides on simulated microgravity-induced functional inhibition of human NK cells Carbohydrate Polymers 3.479 Ting Huyan, et al. 2014 Jan http://www.sciencedirect.com/science/article/pii/S0144861713010321
A fungal protein elicitor PevD1 induces Verticillium wilt resistance in cotton Plant Cell Reports 2.509 Bingwu Bu, et al. 2014 Mar http://link.springer.com/article/10.1007/s00299-013-1546-7
Modulation of HBV replication by microRNA-15b through targeting hepatocyte nuclear factor 1α Nucleic Acids Research 8.278 Xiaopeng Dai, et al. 2014 Apr http://nar.oxfordjournals.org/content/early/2014/04/04/nar.gku260.short
Effects of Exogenous Methyl Jasmonate on the Biosynthesis of Shikonin Derivatives in Callus Tissues of Arnebia euchroma Applied Biochemistry and Biotechnology 1.893 He Hao, et al. 2014 Jun http://link.springer.com/article/10.1007/s12010-014-1025-9
Modulation of modeled microgravity on radiation-induced bystandereffects in Arabidopsis thalianaTing Mutation Research/Fundamental and MolecularMechanisms of Mutagenesis 4 Ting Wanga, et al. 2015 Jan http://dx.doi.org/10.1016/j.mrfmmm.2015.01.010
Arabidopsis BREVIPEDICELLUS Interacts with the SWI2/SNF2 Chromatin Remodeling ATPase BRAHMA to Regulate KNAT2 and KNAT6 Expression in Control of Inflorescence Architecture PLOS Genetics 8.167 Minglei Zhao, et al. 2015 Mar http://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1005125
Arabidopsis histone demethylases LDL1 and LDL2 control primary seed dormancy by regulating DELAY OF GERMINATION 1 and ABA signaling-related genes  Frontiers in Plant Science 3.637 Minglei Zhao, et al. 2015 Mar http://journal.frontiersin.org/article/10.3389/fpls.2015.00159/abstract
Identification and characterization of histonedeacetylasesintomato(Solanumlycopersicum) Frontiers in Plant Science 3.637 Linmao Zhao, et al.  2015 Jan http://journal.frontiersin.org/article/10.3389/fpls.2014.00760/abstract
Identification of miR-2400 gene as a novel regulator in skeletal muscle satellite cells proliferation by targeting MYOG gene Biochemical and Biophysical Research Communications 2.297 Wei Wei Zhang, et al.  2015 Jun http://www.sciencedirect.com/science/article/pii/S0006291X15300358
Characterization of GST genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense Pest Management Science 2.694 Lulu Dai, et al.    2015 Jun http://onlinelibrary.wiley.com/doi/10.1002/ps.4059/abstract
Bitter taste receptor mTas2r105 is expressed in small intestinal villus and crypts Biochemical and Biophysical Research Communications 2.297 Fu Gu, et al.  2015 Jun http://www.sciencedirect.com/science/article/pii/S0006291X15300875
Overexpression of TsApx1 from Thellungiella salsuginea improves abiotic stress tolerance in transgenic Arabidopsis thaliana Biologia Plantarum 1.849 Z. Q. Li, et al. 2015 Jul http://link.springer.com/article/10.1007/s10535-015-0533-y
Isolation of a maize ZmCI-1B promoter and characterization
of its activity in transgenic maize and tobacco
Plant cell reports 3.071 Ye Li, et al.  2015 May http://link.springer.com/article/10.1007%2Fs00299-015-1799-4
Overexpression of ZmIRT1 and ZmZIP3 Enhances Iron and Zinc Accumulation in
Transgenic Arabidopsis
PLOS ONE 3.234 Suzhen Li, et al. 2015 Aug http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0136647
Identification of a novel partial agonist of liver X receptor α (LXRα) via screening Biochemical Pharmacology 4.65 Ni Li, et al. 2014 Dec http://www.ncbi.nlm.nih.gov/pubmed/25450668

 

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