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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
  • Product Name: TransScript All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal )
  • Subtitle:
  • Catalog Number: AT341
  • Storage: at -20℃ for two years
  • 币种:  
    • Specification: 50 rxns×20 µl Systems 100 rxns×20 µlSystems
  • Quantity:  

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Overview

 

Description

The kit provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H2O. Simultaneous genomic DNA removal and cDNA synthesis are performed. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85oC for 5 seconds. The resulting cDNA is suitable for qPCR, not for regular PCR.

 

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for qPCR ready cDNA.
• qPCR ready cDNA in 15 minutes.
• cDNA up to 250 bp.

 

Applications

Multiple copy and low copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer Clinical and Translational Oncology 2.077 J. Guo, et al. 2015 Oct http://link.springer.com/article/10.1007/s12094-015-1305-z
Radiation-Induced Epigenetic Bystander Effects Demonstrated in Arabidopsis Thaliana REGULAR ARTICLES   Wei Xu, et al.  2015 May http://www.rrjournal.org/doi/abs/10.1667/RR13909.1
Perfluorooctane sulfonate induces apoptosis of hippocampal neurons in rat offspring associated with calcium overload Toxicology Research 3.983 Yu Wang, et al. 2015 Mar http://pubs.rsc.org/en/content/articlelanding/2015/tx/c4tx00177j#!divAbstract
Sodic alkaline stress mitigation by exogenous melatonin in tomato needs nitric oxide as a downstream signal Journal of Plant Physiology 2.557 Na Liu, et al. 2015 Jul http://www.sciencedirect.com/science/article/pii/S0176161715001947
Ube2s regulates Sox2 stability and mouse ES cell maintenance Cell Death & Differentiation  8.184 J Wang, et al.  2015  Aug http://www.nature.com/cdd/journal/vaop/ncurrent/full/cdd2015106a.html
An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation.  Mol. Syst. Biol. 14.099 Caihuan Tian, et al. 2014 Nov http://msb.embopress.org/content/10/10/755

 

 

 

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