Your present location:HomeProductsCloning and Mutagenesis SystemCloning Vectors / pEASY®-Blunt Simple Cloning Kit
  • Product Name: pEASY®-Blunt Simple Cloning Kit
  • Catalog Number: CB111
  • Storage: Competent Cell at -70°C for six months; others at -20°C for one year
  • 币种:  
    • Specification: 20 rxns 60 rxns
  • Quantity:  

Quick purchase Purchase Favorite


Overview Contents& storage Citations & references Related Images Download  


pEASY ®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY ® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.
•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•SR primer and M13 forward primer for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.



Contents & storage



CB111-01 (20 rxns)

CB111-02 (60 rxns)

pEASY ®-Blunt Simple Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)



Control Primers (10 μM)



M13 Forward Primer (10 μM)



M13 Reverse Primer (10 μM)

50 µl

150 µl

SR Primer (10 μM)


150 µl

Trans1-T1 Phage Resistant Chemically Competent Cell

10×100 μl



Citations & references
Literature Journal IF Author Date Link
Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis Fungal Diversity 5.319 Yu Z, et al. 2013 Jan
The ammonia oxidizing and denitrifying prokaryotes associated with sponges from different sea areas Microbial Ecology 3.277 Han M, et al. 2013 Aug
Bacterial and archaeal symbionts in the South China Sea sponge Phakellia fusca: community structure, relative abundance, and ammonia-oxidizing populations Marine Biotechnology (NY) 2.739 Han M, et al. 2012 Dec
Low Diversity Bacterial Community and the Trapping Activity of Metabolites from Cultivable Bacteria Species in the Female Reproductive System of the Oriental Fruit Fly, Bactrocera dorsalis Hendel (Diptera: Tephritidae). International Journal of Molecular Sciences 2.598 Shi Z, et al. 2012
Detection and quantification of cultured marine Alexandrium species by real-time PCR World Journal of Microbiology and Biotechnology 1.262 Zhang F, Li Z. 2012 Dec
Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges Microbial Ecology 3.277 Zhou K, et al. 2011 Oct

A novel frame-shift mutation of GLI3 causes non-syndromic and complex digital anomalies in a Chinese family Clinica Chimica Acta 2.85 Cheng F, et al. 2011 May

Modulation of cell wall synthesis and susceptibility to vancomycin by the two-component system AirSR in Staphylococcus aureus NCTC8325 BMC Microbiology 3.104 Haipeng Sun,et al. 2013 Oct

Metallothionein 2 (SaMT2) from Sedum alfredii Hance Confers Increased Cd Tolerance and Accumulation in Yeast and Tobacco. Plos One 3.534 Jie Zhang, et al. 2014 Sep
RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid Organogenesis PLOS ONE 3.73 Bin Gao,et al. 2013 Aug
Polymorphisms of Chicken TLR3 and 7 in Different Breeds PLOS ONE 3.73 Wenke Ruan 2015 Mar
The amino acid residues at 102 and 104 in GP5 of porcine reproductive and respiratory syndrome virus regulate viral neutralization susceptibility to the porcine serum neutralizing antibody Virus Research 2.324 Baochao Fan, et al. 2015 Jun

Cloning and characterization of a novel Nicotiana tabacum ABC transporter involved in shoot branching Physiologia Plantarum 3.138 Xiaodong Xie, et al. 2015 Feb


Construction and evaluation of a fluorescence-based live attenuated Escherichia coli delivery system for generating oral vaccine candidate Applied Microbiology and Biotechnology 3.337 Wenxin Liu, et al. 2015 May
The broad pattern recognition spectrum of the Toll-like receptor in mollusk Zhikong scallop Chlamys farreri Developmental & Comparative Immunology 2.815 Mengqiang Wang, et al. 2015 May

Construction and characterization of an infectious cDNA clone of encephalomyocarditis virus from pigs in China Archives of Virology 2.39 Puxian Fang, et al. 2014 Nov

Identification of two antagonists of the scavenger receptor CD36 using a high-throughput screening model Analytical Biochemistry 2.582 Yanni Xu, et al. 2010 May
Related Images

  To be closed automatically in X seconds

View my favorites

Related products