This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.
•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.