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  • Product Name: pEASY®-Uni Seamless Cloning and Assembly Kit
  • Catalog Number: CU101
  • Storage: Competent Cell at -70°C for six months; others at -20°C for 9 months
  • 币种:  
    • Specification: 10 rxns
  • Quantity:  

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This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.
•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.


Contents & storage




2×Assembly Mix

50 μl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl

Linearized pUC19 Control Vector (10 ng/μl)

3 μl

Control Insert (1 kb,20 ng/μl)

3 μl


Citations & references


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