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  • Product Name: DMT Enzyme
  • Catalog Number: GD111
  • Storage: at -20°C for two years.
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Overview

 Description

DMT cuts the sequence GmATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.

 

 Unit Definition

One unit (U) is defined as the amount of enzyme required for completely digesting 1 μg methylated pBR322 DNA at 37°C for one-hour reaction in a 50 μl reaction system.

 

 Application

In vitro site-directed DNA mutagenesis; degradation of methylated plasmid template

 

Contents& storage

 

Restriction Site:

CH3

5’…GA TC…3’

3’…CT AG…5’

CH3

 

Citations & references

 

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