DMT cuts the sequence GmATC (G is methylated) but does not cut the sequence GATC (G is not methylated). This enzyme cuts DNA prepared from most commonly used E.coli strains (dam+ strain), but does not cut PCR products.
One unit (U) is defined as the amount of enzyme required for completely digesting 1 μg methylated pBR322 DNA at 37°C for one-hour reaction in a 50 μl reaction system.
In vitro site-directed DNA mutagenesis; degradation of methylated plasmid template
GD111 DMT Enzyme
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