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  • Product Name: DNase I (Rnase Free)
  • Catalog Number: GD201
  • Storage: at -20°C for one year.
  • 币种:  
    • Specification: 1500 units
  • Quantity:  

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Overview

 Description

Deoxyribonuclease I (DNase I) is an endonuclease that degrades doubleand single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any site, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.

 Unit Definition

One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.

 Source

Purified from bovine pancreas

 

Contents& storage

Molecular Weight:

32 kDa (monomer)

Activity Test Condition:

40 mM Tris-HCl (pH8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pBR322 DNA

Purity:

Free of other DNA endonucleases and exonucleases, free of RNase

Storage Buffer:

50 mM Tris-acetate (pH 7.5), 10 mM CaCl2, 50% (v/v)glycerol

10×Reaction Buffer:

100 mM Tris-HCl (pH7.5 at 25 °C), 100 mM MgCl2, 1 mM CaCl2

Components:

DNase I (3 units/μl)

1500 units

10×DNase I Reaction Buffer

2×1 ml

200 mM EDTA

1 ml

 

 

 

 

Citations & references

 

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