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Your present location:HomeProductsRestriction Enzyme and Modification EnzymeFast Restriction Enzyme / FlyCut® EcoRI
  • Product Name: FlyCut® EcoRI
  • Catalog Number: JE201
  • Storage: at -20℃ for two years
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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

Concentration 20,000 units/ml

Recognition Site

5’...G↓AATTC...3’

3’...CTTAA↑G...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references
Literature Journal IF Author Date Link
Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence Oncology Letters 1.554 Jian‑Zhi Gao, et al. 2016 Jan
http://www.ncbi.nlm.nih.gov/pubmed/?term=Long+non-coding+RNA+HOTAIR+is+a+marker+for+hepatocellular+carcinoma+progression+and+tumor+recurrence

 

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