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Your present location:HomeProductsRestriction Enzyme and Modification EnzymeFast Restriction Enzyme / FlyCut® PstI
  • Product Name: FlyCut® PstI
  • Catalog Number: JP101
  • Storage: at -20℃ for two years
  • 币种:  
    • Specification: 5000 Units 10000 Units
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Overview
 

Description

FlyCut® PstI is expressed and purified from E.coli that carries the recombinant PstI gene. The molecular weight is 38.3 kDa, with the recognition site at CTGCA^G. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 80oC for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

  Concentration 20,000 units/ml
 

Recognition Site

5’...A↓GATCT...3’

3’...TCTAG↑A...5’

 

Applications

Genomic DNA, plasmid DNA, PCR product 

 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

 

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references

 

 

Related Images

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JP101 FlyCut® PstI
MSDS-JP101

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