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Your present location:HomeProductsRestriction Enzyme / FlyCutTM XbaI
  • Product Name: FlyCutTM XbaI
  • Catalog Number: JX101
  • Storage: at -20℃ for two years
  • 币种:  
    • Specification: 1500 units 3000 units
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Overview

Description

.FlyCutTM XbaI is expressed and purified from E.coli that carries the recombinant XbaI gene. The molecular weight is 24.7 kDa, with the recognition site at T^CTAGA. The reaction is conducted for 5-10 minutes at 37oC, and heat-inactivated at 65oC for 20 minutes. This enzyme is not sensitive to dcm or mammalian CpG methylation, but sensitive to dam methylation.

Concentration 20,000 units/ml

Recognition Site

5’...T↓CTAGA...3’

3’...AGATC↑T...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references

 

 

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