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  • Product Name: EasyPure® miRNA Kit
  • Catalog Number: ER601
  • Storage: LB10 at 4°C in dark for one year, others at room temperature (15°C-25°C) for one year
  • 币种:  
    • Specification: 50 rxns
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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

EasyPure® miRNA Kit provides a simple and fast column based method to isolate small RNA (≤200 nt) from cells, tissues, fresh blood and virus. Samples are lysed with lysis buffer. The addition of chloroform to the sample separates the solution into an upper colorless aqueous phase containing RNA, an interphase and a lower organic phase. High molecular RNA (28S rRNA, 18S rRNA, mRNA) is bound to a silica membrane. Small RNA in the flow-through can be bound to a miRNA spin column.
 

Applications

miRNA related research.

 

Contents& storage

 

Sample Requirement

Material  Amount
Tissue  50-100 mg
Cell 1×107 cells
Fresh Blood 50-200 μl

 

Citations & references

 

Literature Journal IF Author Date Link
PKM2 depletion induces the compensation of glutaminolysis through
β-catenin/c-Myc pathway in tumor cells
Cellular Signalling 4.471 Haili Wu, et al.     2014 Jul http://www.sciencedirect.com/science/article/pii/S0898656814002460

 

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