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  • Product Name: pEASY® -Blunt E1 Expression Kit
  • Catalog Number: CE111
  • Storage: Competent Cell at -70°C for six months; others at -20°C for nine months
  • 币种:  
    • Specification: 10 rxns
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pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
• 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.
• T7 promoter primer and T7 terminator primer for sequencing.
• Bacteriophage T7lac promoter for high level expression.
• N-terminal 6×His tag for easy purification.
• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
• E1 Expression Plasmid included as negative control.


Contents& storage


Citations & references


Literature Journal IF Author Date Link
Identification of a novel class B scavenger receptor homologue in Portunus trituberculatus: Molecular cloning and microbial ligand binding fish & shellfish immunology 3.025 Yang N, et al. 2016 Nov
Genome-wide identification and functional analysis of S-RNase involved in the self-incompatibility of citrus molecular genetics and genomics 2.622 Liang M, et al. 2016 Dec
Characterization of a neutral recombinant xylanase from Thermoactinospora rubra YIM 77501T antonie van leeuwenhoek international journal of general and molecular microbiology 1.944 Yin YR, et al. 2017 Mar


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