ProteinSafeTM Phosphatase Inhibitor Cocktail (100×) is a ready-to-use mixture of fourphosphatase inhibitors (sodium fluoride,sodium pyrophosphate, β-glycerophosphate, and sodium orthovanadate) that has been optimized to protect proteins from being dephosphorylated by endogenous phosphatases, including serine/threonine, tyrosine, acidic and alkaline phosphatases. This cocktail is suitable for use in proteinpurification, Western Blot, Co-IP, ChIP, protein kinase activity assay, etc.
· All-in-one format: a mixture of broad-spectrum phosphatase inhibitors to prevent protein dephosphorylation.
· Ready to use: no need to thaw and dissolve the cocktail, just use directly.
· Compatibility: compatible with most of protein lysis buffers composed of detergent and do not interfere with protein quantitation.