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  • Product Name: TransDetect® Double-Luciferase Reporter Assay Kit
  • Catalog Number: FR201
  • Storage: at -20°C in dark for one year.
  • 币种:  
    • Specification: 50 rxns 200 rxns
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

TransDetect® Double-Luciferase Reporter Assay Kit provides an efficient method to perform dual-reporter assays. In the assay, the activities of firefly (Photinus pyralis) and Renilla (sea pansy) luciferases are measured sequentially from a single sample. The firefly luciferase reporter is measured firstly by adding Luciferase Reaction Reagent to generate a luminescent signal. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated by adding Luciferase Reaction Reagent II to the same tube.

 

Contents& storage

at -20 °C in dark for one year. Prepared luciferase assay reagents (Luciferase Reaction Reagent and Luciferase Reaction Reagent II) should be stored in aliquots in dark at -20°C for one month or at -70°C for one year.

 

Citations & references
Literature Journal IF Author Date Link
Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease CELL DEATH & DISEASE 5.378 Shan W, et al. 2015 Jul http://www.ncbi.nlm.nih.gov/pubmed/26203862
Molecular characterization, transcriptional activity and nutritional regulation of peroxisome proliferator activated receptor gamma in Nile tilapia (Oreochromis niloticus) general and comparative endocrinology 2.667 He AY, et al.  2015 Nov http://www.ncbi.nlm.nih.gov/pubmed/26002036
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