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  • Product Name: TransDetect® Annexin V-FITC/PI Cell Apoptosis Detection Kit
  • Catalog Number: FA101
  • Storage: at 4°C in dark for one year.
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The Annexin V-FITC/PI Cell Apoptosis Kit provides a rapid and sensitive method for early apoptosis detection. In normal cells, the membrane phospholipid phosphatidylserine (PS) is located on the cytoplasmic surface of the membrane. In apoptotic cells, PS is translocated from the inner to the outer leaflet of the plasma membrane, thereby exposing PS to the external cellular environment. The FITC-conjugated Annexin V, a Ca2+ dependent phospholipid-binding protein, can bind specifically to the exposed PS. Propidium iodide (PI) is a nucleic acid binding dye, which binds tightly to the nucleic acids in the cells and stains the cells with red fluorescence.PI is impermeant to live cells and early apoptotic cells, so the combination of Annexin V-FITC and PI staining allows the differentiation among different stage of apoptotic cells and necrosis cells.


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Citations & references
Literature Journal IF Author Date Link
Acetylation of p53 at lysine 120 upregulates Apaf-1 and sensitizes mitochondrial apoptotic pathway JOURNAL OF BIOLOGICAL CHEMISTRY 4.573 Tao Yun, et al. 2016 Feb
Tetracycline-inducible shRNA targeting long non-coding RNA PVT1 inhibits cell growth and induces apoptosis in bladder cancer cells
Oncotarget 6.359 Chengle Zhuang, et al.   2015 Oct
Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum PLOS ONE 3.057 Li S, et al.   2016 Dec
Carboxyamidotriazole inhibits oxidative phosphorylation in cancer cells and exerts synergistic anti-cancer effect with glycolysis inhibition Cancer Letters 5.621 Rui Ju, et al.       2015 Oct
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