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  • Product Name: TransFast Taq DNA Polymerase
  • Subtitle: High Speed Taq DNA Polymerase, 6kb/min
  • Catalog Number: AP101 
  • Storage: at -20 ℃ for two years
  • 币种:  
    • Specification: 500 units 6×500 units 500 Units(With dNTPs) 6×500 Units(With dNTPs)
  • Quantity:  

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Overview

Description

TransFast® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.

Highlights

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

Applications

• Routine PCR
• High throughput PCR
• Colony PCR

Unit Definition

One unit of TransFast® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74.

Quality Control

TransFast® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransFast® Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

10×TransFast® Taq Buffer (with Mg2+)

200 mM Tris-HCl (pH 8.4), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

Contents& storage

Citations & references
Literature Journal IF Author Date
Yes-associated protein (YAP) increases chemosensitivity of hepatocellular carcinoma cells by modulation of p53 Cancer Biology & Therapy 3.287 Nan B, et al.   2013 Jun
Inhibitory Effect of Baicalin on iNOS and NO Expression in Intestinal Mucosa of Rats with Acute Endotoxemia PloS one 3.73 A Feng,et al.  2013 Jun
The Arabidopsis APC4 subunit of the anaphase-promoting complex/cyclosome (APC/C) is critical for both female gametogenesis and embryogenesis The Plant Jounal 6.16 Wang Y, et al. 2012 Jan
Characterization of sequence-related amplified polymorphism markers analysis of tree peony bud sports Scientia Horticulturae 1.396 Xiaoyan Han, et al. 2008 Feb
Berberine ameliorates COX-2 expression in rat small intestinal mucosa partially through PPARγ pathway during acute endotoxemia International Immunopharmacology 2.417 Ai-Wen Feng, et al. 2012 Jan
The complete mitochondrial genome of Cervaphis quercus (Insecta: Hemiptera: Aphididae: Greenideinae) Insect Science 1.786 Yuan Wang, et al. 2014 Jun
Biosynthesis of odd-chain fatty alcohols in Escherichia coli Metabolic Engineering 6.767 Ying-Xiu Cao, et al.     2015 Mar
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