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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR Enzyme / EasyTaq DNA Polymerase for PAGE
  • Product Name: EasyTaq DNA Polymerase for PAGE
  • Subtitle: Special Buffer for PAGE
  • Catalog Number: AP112
  • Storage: at -20°C for two years
  • 币种:  
    • Specification: 2500 units 4×2500 units 2500 Units(With dNTPs) 4×2500 Units(With dNTPs)
  • Quantity:  

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Overview

 

Description

EasyTaq® DNA Polymerase for PAGE is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The
enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase for PAGE
has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. lt lacks 3′-5′ exonuclease activity. This enzyme is supplied with
unique buffer, and its PCR product is suitable for SDS-PAGE and agarose gel electrophoresis.

 

Highlights

• Extension rate is about 1-2 kb/min.
• Unique buffer system compatible with PAGE.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T
vectors.
• Amplification of genomic DNA fragment up to 3 kb.

 

Applications

Short fragment PCR

 

Unit Definition

One unit of EasyTaq® DNA Polymerase for PAGE incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

EasyTaq® DNA Polymerase for PAGE has passed the following quality control assays: functional absence of double- and
single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase for
PAGE has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×EasyTaq® Buffer for PAGE (with Mg2+)

200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

 

Citations & references

 

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