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  • Product Name: TransTaq®-T DNA Polymerase
  • Catalog Number: AP122
  • Storage: at -20 ℃ for two years
  • 币种:  
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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Overview

 

Description

TransTaq®-T DNA Polymerase is a mixture of EasyTaq® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq® DNA Polymerase. It is more suitable for high fidelity TA cloning.

 

Highlights

TransTaq®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.

 

Applications

• Complex templates
• TA cloning

 

Unit Definition

One unit of TransTaq®-T DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

TransTaq®-T DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransTaq®-T DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. 

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×TransTaq®-T Buffer

200 mM Tris-HCl (pH 9.0), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

 

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