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  • Product Name: TransStart Taq DNA Polymerase
  • Subtitle: Hot start Taq DNA polymerase
  • Catalog Number: AP141
  • Storage: at -20 ℃ for two years
  • 币种:  
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
  • Quantity:  

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Overview

 

Description

TransStart® Taq DNA Polymerase is a hot start Taq DNA polymerase containing Taq DNA polymerase and two proprietary DNA binding proteins. At room temperature, one binding protein binds to double-strand DNA template and another binding protein binds to primer. These unique formulations effectively neutralize the DNA polymerase activity at room temperature. Blocking proteins are released from templates and primers during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR. 

 

Highlights

TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.

 

Applications

• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR

 

Unit Definition

One unit of TransStart® Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

TransStart® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. 

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×TransStart® Taq Buffer with 20 mM MgSO4

500 mM Tris-HCl (pH 9.0), 200 mM (NH4)2SO4, 20 mM MgSO4, 10% glycerol, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Improvement of LATE-PCR to allow single-cell analysis by pyrosequencing The Analyst 3.969 Song Q, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/23817386
Isolation and characterization of a Δ1-pyrroline-5-carboxylate synthetase (NtP5CS) from Nitraria tangutorum Bobr. and functional comparison with its Arabidopsis homologue Molecular Biology Reports  2.024 Linlin Zheng,et al.  2014 Jan http://link.springer.com/article/10.1007/s11033-013-2893-8#
Analysis of Genetically Modified Organisms by Pyrosequencing on a Portable Photodiode-based Bioluminescence Sequencer Food Chemistry 3.391 Qinxin Song et,al.    2014 Jan http://www.sciencedirect.com/science/article/pii/S0308814614000065
The Sinorhizobium meliloti ntrX gene is involved in succinoglycan production, motility, and symbiotic nodulation on alfalfa AEM 3.668 Dong Wang et,al.   2013 Sep http://aem.asm.org/content/79/23/7150.short
Improvement of LATE-PCR to allow single-cell analysis by pyrosequencing  Analyst 4.107 Qinxin Song,et,al.     2013 Jun http://pubs.rsc.org/en/content/articlelanding/2013/an/c3an00821e#!divAbstract
Effects of iodine-125 seeds on the methylation of SFRP2 and P16 in colorectal cancer Experimental and Therapeutic Medicine  1.269 Xiaogang Li et,al. 2013 Dec http://www.spandidos-publications.com/etm/6/5/1225?text=fulltext
Retrospective Survey of Museum Specimens Reveals Historically Widespread Presence of Batrachochytrium dendrobatidis in China EcoHealth  2.451 Wei Zhu, et,al.  2014 Jan http://link.springer.com/article/10.1007/s10393-013-0894-7#
Generate Western blot protein marker from a single construct Analytical Biochemistry 2.996 Zhang YM. et al.  2009 Jul  http://www.ncbi.nlm.nih.gov/pubmed/19393217
RcRR1, a Rosa canina Type-A Response Regulator Gene, Is Involved in Cytokinin-Modulated Rhizoid
Organogenesis
PLOS ONE 3.73 Bin Gao,et al 2013 Aug http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0072914
A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization Chemical Communications 6.378 Cui-Yuan Yu, et al 2013 Jul http://pubs.rsc.org/en/content/articlelanding/2013/cc/c3cc44125c#!divAbstract
Global and endemic Asian lineages of the emerging pathogenic fungus Batrachochytrium dendrobatidis widely infect amphibians in China. Diversity and Distributions 6.122 Changming Bai,et al. 2012 Sep

http://onlinelibrary.wiley.com/doi/10.1111/j.1472-4642.2011.00878.x/
abstract;jsessionid=120E1C536EA587D1E0B8DED6ED
295EE8.f04t02?userIsAuthenticated=false&deniedAccessCustomisedMessage=

Enhanced Horizontal Transfer of Antibiotic Resistance Genes in Freshwater Microcosms Induced by an Ionic Liquid PLoS One 3.73 Qing Wang, et al.   2015 May http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126784
Variability in the cathelicidin 6 (CATHL-6) gene in Tianzhu white yak from Tibetan area in China Genetics and Molecular Research 0.775 G.X. E, et al.  2015 Apr http://www.funpecrp.com.br/gmr/year2015/vol14-2/pdf/gmr5473.pdf
A survey for Batrachochytrium salamandrivorans in Chinese amphibians Current Zoology 1.392 Wei ZHU, et al. 2014 http://www.actazool.org/site_media/onlinefirst/downloadable_file
/2014/05/04/Wei_ZHU.pdf

 

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