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  • Product Name: EasyPfu DNA Polymerase
  • Subtitle:
  • Catalog Number: AP211
  • Storage: at -20°C for two years
  • 币种:  
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

EasyPfu DNA Polymerase is an engineered version of pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.

 

Highlights

EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.

 

Applications

• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis

 

Unit Definition

One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers

 

10×EasyPfu Buffer with 20 mM MgSO4

200 mM Tris-HCl (pH 8.8), 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Characterization of a family 5 glycoside hydrolase isolated from the outer membrane of cellulolytic Cytophaga hutchinsonii Applied Microbiology and Biotechnology 3.689 Zhu Y, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/22790541
Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalyzing the synthesis of ethylene in Agaricus bisporus Biochimica et Biophysica Acta 3.848 Meng D, et al. 2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/24016603
Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay Journal of Agricultural and Food Chemistry 2.906 Liu B. et al. 2013 Jan  http://www.ncbi.nlm.nih.gov/pubmed/23317377
Alternatively spliced transcripts of group 3 late embryogenesis abundant protein from Pogonatherum paniceum confer different abiotic stress tolerance in Escherichia coli Journal of Plant Physiology 2.791 Wang WG. et al. 2012 Oct  http://www.ncbi.nlm.nih.gov/pubmed/22902206
Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli PLoS One 3.73 Yang J. et al.  2012 http://www.ncbi.nlm.nih.gov/pubmed/22558074 
Spatial distribution of prokaryotic symbionts and ammoxidation, denitrifier bacteria in marine sponge Astrosclera willeyana Scientific Reports 2.927 Yang Z, Li Z. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22829982 
Detection of pathogenic mutations and the mechanism of a rare chromosomal rearrangement in a Chinese family with Becker muscular dystrophy Clinica Chimica Acta 2.85 Li F, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22910583 
Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3 World Journal of Microbiology and Biotechnology 1.532 Huang X. et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22914897
Discovery of Antagonists for Human Scavenger Receptor CD36 via an ELISA-Like High-Throughput Screening Assay Journal of Biomolecular Screening 2.207 Wang L, et al. 2010 Mar

http://www.ncbi.nlm.nih.gov/pubmed/?term=Discovery%20of%
20Antagonists%20for%20Human%20Scavenger%20Receptor%20CD36%
20via%20an%20ELISA-Like%20High-Throughput%20Screening%20Assay

Ectopic Expression of a Proteinase Inhibitor I4 (MtPiI4) Gene from Medicago truncatula Confers Plant Resistance to Pseudomonas syringae pv. Tomato DC3000 Plant Molecular Biology Reporter   Di Sun, et al.     2015 Mar http://link.springer.com/article/10.1007/s11105-015-0865-y
Transcriptome-Wide Identification of Salt-Responsive Members of the WRKY Gene Family in Gossypium aridum PLoS One 3.534 Xinqi Fan, et al. 2015 May http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0126148
Identification of UshA as a major enzyme for NAD degradation in Escherichia coli Enzyme and Microbial Technology 2.592 Lei Wang, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S0141022914000544

 

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