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  • Product Name: EasyPfu® DNA Polymerase
  • Catalog Number: AP211
  • Storage: at -20°C for two years
  • 币种:  
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
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EasyPfu DNA Polymerase is an engineered version of Pfu DNA Polymerase with enhanced yield and higher fidelity. EasyPfu DNAPolymerase possesses a proofreading 3'-5' exonuclease activity.



EasyPfu DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 0.5 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 6 kb.
• Amplification of plasmid DNA fragment up to 10 kb.



• High fidelity PCR
• Blunt-end cloning
• Site-directed mutagenesis


Unit Definition

One unit of EasyPfu DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.


Quality Control

EasyPfu DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity, >99% homogeneous measured by SDS-PAGE. Each batch of EasyPfu DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.


Storage Buffer

50 mM Tris-HCl (pH 8.0), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) glycerol stabilizers


10×EasyPfu Buffer with 20 mM MgSO4

200 mM Tris-HCl (pH 8.8), 100 mM (NH4)2SO4, 100 mM KCl, 20 mM MgSO4, others



Contents & storage



Citations & references


Literature Journal IF Author Date Link
Characterization of a family 5 glycoside hydrolase isolated from the outer membrane of cellulolytic Cytophaga hutchinsonii Applied Microbiology and Biotechnology 3.689 Zhu Y, et al. 2013 May
Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalyzing the synthesis of ethylene in Agaricus bisporus Biochimica et Biophysica Acta 3.848 Meng D, et al. 2013 Sep
Detection of the Soybean Allergenic Protein Gly m Bd 28K by an Indirect Enzyme-Linked Immunosorbent Assay Journal of Agricultural and Food Chemistry 2.906 Liu B. et al. 2013 Jan
Alternatively spliced transcripts of group 3 late embryogenesis abundant protein from Pogonatherum paniceum confer different abiotic stress tolerance in Escherichia coli Journal of Plant Physiology 2.791 Wang WG. et al. 2012 Oct
Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli PLoS One 3.73 Yang J. et al.  2012 
Spatial distribution of prokaryotic symbionts and ammoxidation, denitrifier bacteria in marine sponge Astrosclera willeyana Scientific Reports 2.927 Yang Z, Li Z. 2012 
Detection of pathogenic mutations and the mechanism of a rare chromosomal rearrangement in a Chinese family with Becker muscular dystrophy Clinica Chimica Acta 2.85 Li F, et al. 2012 Dec 
Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3 World Journal of Microbiology and Biotechnology 1.532 Huang X. et al. 2012 Dec
Discovery of Antagonists for Human Scavenger Receptor CD36 via an ELISA-Like High-Throughput Screening Assay Journal of Biomolecular Screening 2.207 Wang L, et al. 2010 Mar

Ectopic Expression of a Proteinase Inhibitor I4 (MtPiI4) Gene from Medicago truncatula Confers Plant Resistance to Pseudomonas syringae pv. Tomato DC3000 Plant Molecular Biology Reporter   Di Sun, et al.     2015 Mar
Transcriptome-Wide Identification of Salt-Responsive Members of the WRKY Gene Family in Gossypium aridum PLoS One 3.534 Xinqi Fan, et al. 2015 May
Identification of UshA as a major enzyme for NAD degradation in Escherichia coli Enzyme and Microbial Technology 2.592 Lei Wang, et al. 2014 May


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