transbionovo

+86-10-57815027      
中文版 | ENGLISH

Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR Enzyme / TransStart FastPfu Fly DNA Polymerase
  • Product Name: TransStart FastPfu Fly DNA Polymerase
  • Subtitle: Ultra Fast/High Fidelity, Blunt-end Amplicon
  • Catalog Number: AP231 
  • Storage: at -20 ℃ for two years
  • 币种:  
    • Specification: 250 units 500 units 6×500 units 250 Units(With dNTPs) 500 Units(With dNTPs) 6×500 Units(With dNTPs)
  • Quantity:  

Quick purchase Purchase Favorite

 

Overview Contents& storage Citations & references Related Images Download  
Overview

Description

TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfu Fly DNA Polymerase has an xtension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.

 

Highlights

TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

 

Applications

• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

 

Unit Definition

One unit of TransStart® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

TransStart® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol

 

TransStart® FastPfu Fly Buffer

100 mM Tris-SO4 pH 9.2, 50 mM (NH4)2SO4, 200 mM KCl; 10 mM MgSO4; 10% Glycerol; others

 

Contents& storage

Citations & references
Literature Journal IF Author Date Link
The GADD45A (1506T>C) Polymorphism Is Associated with Ovarian Cancer Susceptibility and Prognosis Plos one 3.234 Cunzhong Yuan, et al. 2015 Sep  http://www.plosone.org/article/Authors/info:doi/10.1371
/journal.pone.0138692

 

Related Images

  To be closed automatically in X seconds

View my favorites

Related products
no such related products!