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Mutagenesis System

Category Description :

Partially overlapping primers were used into  PCR amplification, and the amplified products were visible by gel electrophoresis. The amplified products were ring-shaped and easy to be transformed.The mutant clones were screened by DMT enzyme digestion in vitro by degrading non-mutant plasmid template (methylated plasmid template) and degrading non-mutant plasmid template (methylated plasmid template)by  DMT competent cells in vivo .

Mutagenesis System
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