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  • Product Name: EasyTaq® DNA Polymerase
  • Catalog Number: AP111
  • Date: 2016-09-30
  • Views: 47
  • Sold amount:0Piece
    • Specification: 500 units 6×500 units 10×5000 units 4×2500 units 500 Units 6×500 Units 4×2500 Units
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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

EasyTaq® DNA Polymerase is purified from E. coli expressing a cloned DNA polymerase from Thermus aquaticus. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. EasyTaq® DNA Polymerase has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity. It lacks 3′-5′ exonuclease activity. EasyTaq® DNA Polymerase is suitable for routine amplification. PCR products are unsuitable for PAGE.

 

Highlights

• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.

 

Applications

• Routine PCR
• Colony PCR

 

Unit Definition

One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74℃.

 

Quality Control

EasyTaq® DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

 

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

 

10×EasyTaq® Buffer (with Mg2+)

200 mM Tris-HCl (pH 8.3), 200 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, others

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Lack of Structural Variation but Extensive Length Polymorphisms and Heteroplasmic Length Variations in the Mitochondrial DNA Control Region of Highly Inbred Crested Ibis, Nipponia nippon PLoS One 3.73 He XL, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23805212
MACRO: A Combined Microchip-PCR and Microarray System for High-throughput Monitoring of Genetically Modified Organisms ACS 12.113 Ning Shao,et al.   2013 Dec http://pubs.acs.org/doi/abs/10.1021/ac403630a
Evolutionary relationships of Pemphigus and allied genera (Hemiptera: Aphididae: Eriosomatinae) and their primary endosymbiont, Buchnera aphidicola Insect Science 2.144 L Liu et al.      2014 Jan http://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12113/abstract
Detection of Roundup Ready soybean by loop-mediated isothermal amplification combined with a lateral-flow dipstick Food Control 2.806 Xiumin Wang et al.      2013 Jan http://www.sciencedirect.com/science/article/pii/S0956713512003271
Molecular diversity of class 2 integrons in antibiotic-resistant gram-negative bacteria found in wastewater environments in China Ecotoxicology 2.706 Ruirui Xia et al. 2013 Mar http://link.springer.com/article/10.1007/s10646-012-1034-9#
Hydrogen Sulfide Alleviates Postharvest Senescence of Broccoli by Modulating Antioxidant Defense and Senescence-Related Gene Expression J AGR FOOD CHEM  2.912 Shi-Ping Li et al 2014 Jan http://pubs.acs.org/doi/abs/10.1021/jf4047122
Identification of Plasmid-Mediated Quinolone Resistance qnr Genes in Multidrug-Resistant Gram-Negative Bacteria from Hospital Wastewaters and Receiving Waters in the Jinan Area, China Microbial Drug Resistance 2.49 Ruirui Xia et al.  2013 Jul http://online.liebertpub.com/doi/abs/10.1089/mdr.2012.0210
Population genetics of Sargassum horneri (Fucales, Phaeophyta) in China revealed by ISSR and SRAP markers Chinese Journal of Oceanology and Limnology 0.657 Shenhui Yu et al.   2013 May http://link.springer.com/article/10.1007/s00343-013-2064-8#
Molecular diversity and distribution of anammox community in sediments of the Dongjiang River, a drinking water source of Hong Kong Journal of Applied Microbiology 2.479 W.sun et,el.   2013 Nov http://onlinelibrary.wiley.com/doi/10.1111/jam.12367/abstract?deniedAccessCustomised
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Rapid Diversification of FoxP2 in Teleosts through Gene Duplication in the Teleost-Specific Whole Genome Duplication Event PloS one 3.73 Xiaowei Song et al.      2013 Dec http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0083858
Generic status, circumscription, and allopolyploid origin of Faberia (Asteraceae: Cichorieae) as revealed by ITS and chloroplast DNA sequence data Taxon 3.299 Liu, Ying, et al.     2013 Dec

http://www.ingentaconnect.com/content/iapt/tax/2013/00000062/00000006/art00010

Resistance to the Novel Fungicide Pyrimorph in Phytophthora capsici: risk assessment and detection of point mutations in CesA3 that confer resistance PLoS One 3.73 Pang Z, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23431382
Transcriptome-wide identification and characterization of microRNAs from castor bean (Ricinus communis L.) PLoS One 3.73 Xu W, et al. 2013 Jul  http://www.ncbi.nlm.nih.gov/pubmed/23894571

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