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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / EasyScript Reverse Transcriptase
  • Product Name: EasyScript Reverse Transcriptase
  • Catalog Number: AE101
  • Date: 2015-11-05
  • Views: 43
  • Sold amount:0Piece
    • Specification: 10000 units 5×10000 units
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Overview

 

Description

EasyScript® Reverse Transcriptase is an engineered version of M-MLV reverse transcriptase with deficient RNase H activity. The enzyme is purified to near homogeneity from E.coli containing the modified M-MLV RT gene.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 8 kb.

 

Applications

• First-strand cDNA synthesis
• Multiple copy gene detection

 

Unit Definition

One unit of EasyScript® RT incorporates 1 nmol of deoxyribonucleotide into acid precipitable material in 10 minutes at 37oC using Poly(A)/Oligo(dT) as template/primer.

 

5×ES RT Buffer

375 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways Acta Pharmacologica Sinica 2.354 Meng Z, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23645013
Cloning and Expression Analysis of Wheat Pheophorbide a Oxygenase Gene TaPaO Plant Molecular Biology Reporter 5.319 Ma N, et al. 2012 Oct  http://link.springer.com/article/10.1007/s11105-012-0443-5
Synergistic antitumor effects of endostar in combination with oxaliplatin via inhibition of HIF and CXCR4 in the colorectal cell line SW1116 PLoS One 3.23 Jin F. et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23071744
Complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of Sugarcane mosaic virus Virus Genes 1.769 Gao B, et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/21833715
Boty-like retrotransposons in the filamentous fungus Botrytis cinerea contain the additional antisense gene brtn Virology 3.367 Zhao M, et al. 2011 Sep  http://www.ncbi.nlm.nih.gov/pubmed/21802103
Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules Cell Research 10.526 Li Y. et al. 2011 Jan http://www.ncbi.nlm.nih.gov/pubmed/20956998
Molecular cloning and characterization of four heat shock protein genes from Macrocentrus cingulum (Hymenoptera: Braconidae).  Molecular Biology Reports 2.506 Xu P, et al. 2010 Jun http://www.ncbi.nlm.nih.gov/pubmed/19685164
Apis mellifera has two isoforms of cytoplasmic HSP90 Insect Molecular Biology 2.529 Xu PJ, et al. 2010 Aug http://www.ncbi.nlm.nih.gov/pubmed/20522122 
Expression and evolutionary divergence of the non-conventional olfactory receptor in four species of fig wasp associated with one species of fig BMC Evolutionary Biology 3.521 Lu B. et al. 2009 Feb  http://www.ncbi.nlm.nih.gov/pubmed/19232102
Effects of carbon nanotubes on atrazine biodegradation by Arthrobacter sp Journal of Hazardous Materials 4.529 Chengdong Zhang, et al. 2015 Jan http://www.sciencedirect.com/science/article/pii/S0304389415000412
Association of suboptimal health status with psychosocial stress, plasma cortisol and mRNA expression of glucocorticoid receptor α/β in lymphocyte Stress 2.715 Yu-Xiang Yan, et al.  2014 May http://www.tandfonline.com/doi/abs/10.3109/10253890.2014.999233
Resistance of Stenotrophomonas maltophilia to Fluoroquinolones: Prevalence in a University Hospital and Possible Mechanisms Int. J. Environ. Res. Public Health  2.063 Wei Jia, et al.  2015 May http://www.mdpi.com/1660-4601/12/5/5177/htm
Anthrax lethal toxin suppresses high glucose induced VEGF over secretion through a post-translational mechanism International Journal of Ophthalmology 0.705 Wei-Wei Zhang, et al.  2015 Jun http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458645/
Differential Gene Expression in Ovaries of Qira Black Sheep and Hetian Sheep Using RNA-Seq Technique PLoS One  3.23 Han Ying Chen, et al.  2015 Mar http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0120170
Effects of Foxp3 gene modified dendritic cells on mouse
corneal allograft rejection
Int J Clin Exp Med    Yu-Bo Gong, et al.  2015 Mar http://www.ijcem.com/files/ijcem0004267.pdf
A Splice Mutation and mRNA Decay of EXT2 Provoke Hereditary Multiple Exostoses PLoS One 3.23 Chen Tian, et al. 2014 Apr http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.
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