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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / TransScript II Reverse Transcriptase
  • Product Name: TransScript II Reverse Transcriptase
  • Catalog Number: AH101
  • Date: 2015-11-05
  • Views: 19
  • Sold amount:0Piece
    • Specification: 10000 units
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.
• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.

 

Applications

• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

Unit Definition

One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

 

10×TS II RT Buffer

500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Exogenous ARC down-regulates caspase-3 expression and inhibits apoptosis of broiler chicken cardiomyocytes exposed to hydrogen peroxide Avian Pathology 1.729 Wu L, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23391179
Seed size is determined by the combinations of the genes controlling different seed characteristics in rice Theoretical and Applied Genetics 3.658 Yan S, et al. 2011 Nov http://www.ncbi.nlm.nih.gov/pubmed/21805338
Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin
 
Experimental
and Therapeutic
Medicine
1.269 Hai‑Cheng Gao, et al.  2015 Jan http://www.spandidos-publications.com/etm/9/4/1103?text=fulltext
Characterization of the PRMT gene family in rice reveals conservation of arginine methylation PLoS One 3.23 Ahmad A, et al. 2011 http://www.ncbi.nlm.nih.gov/pubmed/21853042

 

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