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  • Product Name: TransScript® II Reverse Transcriptase
  • Catalog Number: AH101
  • Date: 2015-11-05
  • Views: 117
  • Sold amount:0Piece
    • Specification: 10000 units
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TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.

• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.



• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template


Unit Definition

One unit of TransScript® II RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.


10×TS II RT Buffer

500 mM KCl, 30 mM MgCl2, 100 mM Tris-HCl pH 8.4



Contents& storage

Kit Contents


Citations & references


Literature Journal IF Author Date Link
Exogenous ARC down-regulates caspase-3 expression and inhibits apoptosis of broiler chicken cardiomyocytes exposed to hydrogen peroxide Avian Pathology 1.729 Wu L, et al. 2013 Feb
Seed size is determined by the combinations of the genes controlling different seed characteristics in rice Theoretical and Applied Genetics 3.658 Yan S, et al. 2011 Nov
Role of tissue transglutaminase in the pathogenesis of diabetic cardiomyopathy and the intervention effect of rutin
and Therapeutic
1.269 Hai‑Cheng Gao, et al.  2015 Jan
Characterization of the PRMT gene family in rice reveals conservation of arginine methylation PLoS One 3.23 Ahmad A, et al. 2011


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