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  • Product Name: TransScript® II First-Strand cDNA Synthesis SuperMix
  • Catalog Number: AH301
  • Date: 2015-11-05
  • Views: 100
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    • Specification: 50 rxns×20 µl Systems 100 rxns×20 µlSystems
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TransScript® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® II RT/RI Enzyme Mix and 2×TS II Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.






• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template



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Citations & references


Literature Journal IF Author Date Link
The HD-GYP Domain Protein RpfG of Xanthomonas oryzae pv. oryzicola Regulates Synthesis of Extracellular Polysaccharides that Contribute to Biofilm Formation and Virulence on Rice PLoS One 3.23 Zhang Y, et al. 2013
Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4 Biochemical and Biophysical Research Communications 2.406 Li H, et al. 2013 Nov
Desflurane accelerates neuronal cytotoxicity of Aβ by downregulating miR-214 Neuroscience Letters 2.026 Yu Y, Zhang Y. 2013 Sep
Saturated fatty acid palmitate-induced insulin resistance is accompanied with myotube loss and the impaired expression of health benefit myokine genes in C2C12 myotubes Lipids in Health and Disease  2.015 Yang M, et al. 2013 Jul
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.23 Wang B. et al. 2013
Isoflurane Increases Neuronal Cell Death Vulnerability by Downregulating miR-214 PLoS One 3.23 Yan H, et al. 2013
Nitric Oxide Mediates Cytokinin Functions in Cell Proliferation and Meristem Maintenance in Arabidopsis Molecular Plant 6.126 Shen Q, et al. 2013 Jul
An improved CTAB-ammonium acetate method for total RNA isolation from cotton Phytochemical Analysis 2.48 Zhao L, et al. 2012 Nov
Tunneling-nanotube direction determination in neurons and astrocytes Cell Death and Disease 6.044 Sun X, et al. 2012 Dec
GABA attenuates amyloid toxicity by downregulating its endocytosis and improves cognitive impairment Journal of Alzheimer's Disease 4.174 Sun X, et al. 2012
Role of nuclear receptor coactivator 3 (ncoa3) in pluripotency maintenance Journal of Biological Chemistry 4.773 Wu Z, et al. 2012 Nov
Murine myeloid dendritic cells that phagocytose apoptotic T cells inhibit the immune response via NO PLoS One 3.23 Zhong K, et al. 2012
Tunneling-nanotube development in astrocytes depends on p53 activation Cell Death and Differentiation 8.371 Wang Y, et al. 2011 Apr
Obligate mutualism within a host drives the extreme specialization of a fig wasp genome Genome Biology 10.288 Jin-Hua Xiao,et al. 2013 Dec
Morphine protects against intracellular amyloid toxicity by inducing estradiol release and upregulation of Hsp70 The Journal of Neuroscience 6.908 Cui J, et al. 2011 Nov
Growth and stress responses of the earthworm Eisenia fetida to Escherichia coli O157:H7 in an artificial soil Microbial Pathogenesis 1.974 Liu X, et al. 2009 May
The Batten disease gene CLN3 confers resistance to endoplasmic reticulum stress induced by tunicamycin Biochemical and Biophysical Research Communications 2.406 Dan Wu, et al. 2014 Apr
Adaptive evolution of the circadian gene timeout in insects Scientific Reports 2.927 Haifeng Gu, et al. 2014 Feb



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