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  • Product Name: TransScript II One-Step gDNA Removal and cDNA Synthesis SuperMix
  • Catalog Number: AH311
  • Date: 2015-11-05
  • Views: 10
  • Sold amount:0Piece
    • Specification: 50 rxns×20 µl Systems 100 rxns×20 µlSystems
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Overview

 

Description

Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA removerand reverse transcriptase are inactivated by heating at 85oC for 5 seconds.

 

Highlights

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 15 kb.

 

Applications

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
miR-888 regulates side population properties and cancer metastasis in breast cancer cells Biochemical and Biophysical Research Communications 2.406 Shengjian Huang, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S0006291X14008766
Effect of amino acid mutation at position 127 in 3A of a rabbit-attenuated foot-and-mouth disease virus serotype Asia1 on viral replication and infection Virologica Sinica 1.28 Aiguo Xin, et al. 2014 Oct http://link.springer.com/article/10.1007/s12250-014-3464-4
Molecular characterization and expression profiling of novel laccase isoenzyme genes from Fusarium solani X701, a wood-rotting ascomycete International Biodeterioration & Biodegradation 2.131 ZhaoWei Wu, et al. 2015 Jun http://www.sciencedirect.com/science/article/pii/S096483051500178X
Wheat bHLH transcription factor gene, TabHLH060, enhances susceptibility of transgenic Arabidopsis thaliana to Pseudomonas syringae Physiological and Molecular Plant Pathology 1.407 Fengtao Wang, et al. 2015 May http://www.sciencedirect.com/science/article/pii/S0885576515000363
TaNAC1 acts as a negative regulator of stripe rust resistance in wheat, enhances susceptibility to Pseudomonas syringae, and promotes lateral root development in transgenic Arabidopsis thaliana Front Plant Sci. 3.948 Fengtao Wang, et al.  2015 Feb http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4342887/
Molecular characterizationofanovelhypovirusfromtheplant
pathogenicfungus Fusarium graminearum
Virology 3.321 Pengfei Li, et al. 2015 Jul http://www.sciencedirect.com/science/article/pii/S0042682215001129
LcMKK, a novel group A mitogen-activated protein kinase kinase gene in Lycium chinense, confers dehydration and drought tolerance in transgenic tobacco via scavenging ROS and modulating expression of stress-responsive genes Plant Growth Regulation 1.672 Dianyun Wu, et al. 2015 Jul http://link.springer.com/article/10.1007/s10725-014-9998-5
LcKRP, a cyclin-dependent kinase inhibitor from Lycium chinense, is involved in an ABA-dependent drought stress-signaling pathway Plant and Soil 2.638 Chunfeng Guan, et al. 2014 May http://link.springer.com/article/10.1007/s11104-014-2134-5

 

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