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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR
  • Product Name: TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR
  • Catalog Number: AH321
  • Date: 2015-11-05
  • Views: 12
  • Sold amount:0Piece
    • Specification: 50 rxns×20 µl Systems
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Overview

 

Description

TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA is suitable for regular PCR, not for qPCR.

 

Highlights

• One-tube format for simple and fast setup and reduced pipetting variability.
• The optimal ratio of oligo(dT)20 primer to random primer(N9) for PCR ready cDNA.
• PCR ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 15 kb.

 

Applications

• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template

 

 

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