TransScript® II Two-Step RT-PCR SuperMix performs first-strand cDNA synthesis and PCR in two steps. 5×TransScript® II All-in-One SuperMix for PCR is used for reverse transcription and 2×TransTaq® HiFi PCR SuperMix II is used for PCR.
Amplification of fragment up to 15 kb.
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
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