One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript® II RT and TransTaq® HiFi DNA Polymerase are used in the kit.
Amplification of fragment up to 8 kb.
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
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