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  • Product Name: TransStart Green qPCR SuperMix
  • Catalog Number: AQ101
  • Date: 2015-11-05
  • Views: 41
  • Sold amount:0Piece
    • Specification: 1 ml 5×1 ml 15×1 ml
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Overview

 

Description

TransStart® Green qPCR SuperMix is a ready-to-use qPCR cocktail containing all components, except primer and template. It contains TransStart® Taq DNA Polymerase, SYBR Green I, dNTPs, PCR enhancer and stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH2O.

 

Highlights

• TransStart® Taq DNA Polymerase, hot start with double blocking technique, improves sensitivity, enhances specificity and generates more accurate data.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

 

Applications

N

 

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q

 

 

Contents& storage

 

 

Citations & references

 

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VscO, a putative T3SS chaperone escort of Vibrio alginolyticus, contributes to virulence in fish and is a target for vaccine development Fish & Shellfish Immunology 2.964 Zhou Z, et al. 2013 Nov http://www.ncbi.nlm.nih.gov/pubmed/23994282
Proteomic comparison of the probiotic bacterium Lactobacillus casei Zhang cultivated in milk and soy milk Journal of Dairy Science 2.566 Wang J, et al. 2013 Sep http://www.ncbi.nlm.nih.gov/pubmed/23871367
12-Deoxyphorbol 13-palmitate mediated cell growth inhibition, G2-M cell cycle arrest and apoptosis in BGC823 cells European Journal of Pharmacology 2.592 Xu HY, et al. 2013 Jan  http://www.ncbi.nlm.nih.gov/pubmed/23220710
Expression and immunogenicity analysis of accessory colonization factor A from Vibrio alginolyticus strain HY9901 Fish & Shellfish Immunology 2.964 Cai SH, et al. 2013 Feb http://www.ncbi.nlm.nih.gov/pubmed/23261505
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.23 Wang B. et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23342036
An S-domain receptor-like kinase OsSIK2 confers abiotic stress tolerance and delays dark-induced leaf senescence in rice Plant Physiology 6.555 Chen LJ, et al. 2013 Oct  http://www.ncbi.nlm.nih.gov/pubmed/24143807
Silencing of periostin inhibits nicotine-mediated tumor cell growth and epithelial-mesenchymal transition in lung cancer cells Molecular Medicine Reports 1.17 Wu SQ, et al. 2013 Mar http://www.ncbi.nlm.nih.gov/pubmed/23314871
Decreased expression of SOX6 confers a poor prognosis in hepatocellular carcinoma Cancer Epidermiology 2.232 Guo X, et al. 2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/23731550
Cloning and characterization of the dopamine like receptor in the oyster Crassostrea angulata: expression during the ovarian cycle Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2.069 Yang B, et al. 2013 Mar http://www.ncbi.nlm.nih.gov/pubmed/23274282
Identification and differential expression of two dehydrin cDNAs during maturation of Jatropha curcas seeds Biochemistry (Moscow) 1.149 Omar SA, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/238481510
Tunneling-nanotube direction determination in neurons and astrocytes Cell Death and Disease 6.044 Sun X, et al. 2012 Dec  http://www.ncbi.nlm.nih.gov/pubmed/23222508
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction PLoS One 3.23 Long G, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23236408
Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activity Journal of Cellular Biochemistry 3.062 Xu X, et al.  2012 Jul http://www.ncbi.nlm.nih.gov/pubmed/22573553
Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions Future Microbiology 4.018 Qu Y, et al. 2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22439729
Cloning and expression analysis of recombination activating genes (RAG1/2) in red snapper (Lutjanus sanguineus).  Fish & Shellfish Immunology 2.964 Zhang XL, et al. 2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22266137
The therapeutic effects of daphnetin in collagen-induced arthritis involve its regulation of Th17 cells International Immunopharmacology 2.417 Tu L. et al. 2012 Aug http://www.ncbi.nlm.nih.gov/pubmed/22554834
Effects of low temperatures on proliferation-related signaling pathways in the hippocampus after traumatic brain injury Experimental Biology and Medicine 2.803 Wang L, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23354401
The tumor suppressor role of Src homology phosphotyrosine phosphatase 2 in hepatocellular carcinoma Journal of Cancer Research and Clinical Oncology 2.914 Jiang C, et al. 2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22228034
Pyrosequencing of Haliotis diversicolor transcriptomes: insights into early developmental molluscan gene expression PLoS One 3.73 Huang ZX, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23236463
Stable expression of Y-box protein 1 gene in early development of the abalone Haliotis diversicolor The International Journal of Developmental Biology 2.614 Chen J, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22689369
SOX2 gene regulates the transcriptional network of oncogenes and affects tumorigenesis of human lung cancer cells PLoS One 3.23 Chen S, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22615765
Oral vaccination with attenuated Salmonella enterica serovar Typhimurium expressing Cap protein of PCV2 and its immunogenicity in mouse and swine models Veterinary Microbiology 3.127 Xu XG, et al. 2012 Jun  http://www.ncbi.nlm.nih.gov/pubmed/22326539
Overexpression of lysine specific demethylase 1 predicts worse prognosis in primary hepatocellular carcinoma patients World Journal of Gastroenterology 2.547 Zhao ZK, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23236241
Effect of hypaconitine combined with liquiritin on the expression of calmodulin and connexin43 in rat cardiac muscle in vivo Journal of Pharmacy and Pharmacology 2.033 Yi M, et al. 2012 Nov http://www.ncbi.nlm.nih.gov/pubmed/23058053
Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells Molecular Medicine Reports 1.17 Huang XF, et al. 2012 Sep http://www.ncbi.nlm.nih.gov/pubmed/22751806
Nucleofection of a DNA vaccine into human monocyte-derived dendritic cells Cellular Immunology 1.743 Xie Q, et al. 2012 Mar http://www.ncbi.nlm.nih.gov/pubmed/22632899
Side-effects of resveratrol in HepG2 cells: Reduced pten and increased bcl-xl mRNA expression Molecular Medicine Reports 1.17 Zheng M, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22971900
 Expression features of SOX9 associate with tumor progression and poor prognosis of hepatocellular carcinoma Diagn Pathol 1.85 Guo X, et al.  2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22515642
Molecular characterization and functional analysis of adrenergic like receptor during larval metamorphosis in Crassostrea angulata Aquaculture 2.009 Yang B, et al. 2012 Nov http://www.sciencedirect.com/science/article/pii/S0044848612005212
PKHD1 post‐transcriptionally modulated by miR‐365‐1 inhibits cell‐cell adhesion Cell Biochemistry and Function 1.854 Duan J, et al. 2012 Jul http://www.ncbi.nlm.nih.gov/pubmed/22411058
Cellular tropism, population dynamics, host range and taxonomic status of an aphid secondary symbiont, SMLS (Sitobion miscanthi L type symbiont).  PLoS One 3.23 Li T, et al. 2011 http://www.ncbi.nlm.nih.gov/pubmed/21789197
MiR-124 regulates early neurogenesis in the optic vesicle and forebrain, targeting NeuroD1 Nucleic Acids Research 8.278 Liu K, et al. 2011 Apr http://www.ncbi.nlm.nih.gov/pubmed/21131276
Morphine protects against intracellular amyloid toxicity by inducing estradiol release and upregulation of Hsp70 The Journal of Neuroscience 6.908 Cui J, et al. 2011 Nov  http://www.ncbi.nlm.nih.gov/pubmed/22072674
Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCR BMC Molecular Biology 2.857 Li L. et al. 2011 Feb  http://www.ncbi.nlm.nih.gov/pubmed/21324211
Identification and expression analysis of two small heat shock protein cDNAs from developing seeds of biodiesel feedstock plant Jatropha curcas Plant Science  2.922 Omar SA, et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/21958704
NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis The Plant Jounal 6.16 Zhang B, et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/21801253
Effect of strychnine hydrochloride on liver cytochrome P450 mRNA expression and monooxygenase activities in rat Acta Pharmaceutica Sinica B 2.354 Gao Q, et al. 2011 Aug http://www.sciencedirect.com/science/article/pii/S2211383511000475
PPARγ co-activator-1α co-activates steroidogenic factor 1 to stimulate the synthesis of luteinizing hormone and aldosterone The Biochemical Journal  4.654 Zhu L, et al. 2010 Dec  http://www.ncbi.nlm.nih.gov/pubmed/21108604
MicroRNA-126 modulates the tumor microenvironment by targeting calmodulin-regulated spectrin-associated protein 1 (Camsap1) International Journal of Oncology 2.657 Xin Sun, et al. 2014 May http://www.spandidos-publications.com/ijo/44/5/1678?text=fulltext
Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20 FEMS Microbiology Letters 2.049 Xiaoming Pu, et al. 2014 May http://onlinelibrary.wiley.com/doi/10.1111/1574-6968.12461/abstract;jsessionid=
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Short-term effects of Dechlorane Plus on the earthworm Eisenia fetida determined by a systems biology approach Journal of Hazardous Materials 3.925 Liujun Zhang, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S0304389414001940
Cyclosporine A protects podocytes via stabilization of cofilin-1 expression in the unphosphorylated state Experimental Biology and Medicine 2.803 Xiaoyan Li, et al. 2014 Apr http://ebm.sagepub.com/content/early/2014/04/15/1535370214530365.abstract
Anoctamin 1 calcium-activated chloride channel downregulates estrogen production in mouse ovarian granulosa cells Endocrinology 4.717 Meiyan Sun, et al. 2014 May http://press.endocrine.org/doi/abs/10.1210/en.2013-2155
Short-term effects of Dechlorane Plus on the Earthworm Eiseniafetidadetermined by a systems biology approach Journal of Hazardous Materials 3.925 Liujun Zhang, et al. 2014 Mar http://www.sciencedirect.com/science/article/pii/S0304389414001940
Global Analysis of Ankyrin Repeat Domain C3HC4-Type
RING Finger Gene Family in Plants
PLOS ONE 3.23 Xiaowei Yuan, et al. 2013 Mar http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0058003
Tembusu virus infection in Cherry Valley ducks: The effect ofage at infection Veterinary Microbiolog 3.127 X Y Sun, et al.  2014 Jan http://www.sciencedirect.com/science/article/pii/S0378113513004768
Expression and Functional Analysis of a Pathogenesis-Related Protein 1 Gene, TcLr19PR1, Involved in Wheat Resistance Against Leaf Rust Fungus Plant Molecular Biology Reporter 1.656 Lin Gao et al. 2014 Sep http://link.springer.com/article/10.1007/s11105-014-0790-5
The miR-30 family inhibits pulmonary vascular hyperpermeability in the premetastatic phase by direct targeting of Skp2 in mice Clinical Cancer Research 8.722 Feifei Qi, et al. 2015 Mar http://clincancerres.aacrjournals.org/content/early/2015/03/25/1078-0432.CCR-14-2785.short
New features of triacylglycerol biosynthetic pathways of peanut seeds in early developmental stages Functional & Integrative Genomics 2.479 Mingli Yu, et al. 2015 Jun http://link.springer.com/article/10.1007/s10142-015-0447-y
Effects of Lycium barbarum polysaccharides on oxidative stress in hyperlipidemic mice following chronic composite psychological stress intervention Molecular Medicine
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Isolation and Functional Analysis of SpWOX13 from Stipa purpurea Plant Molecular Biology Reporter 1.656 Xudong Sun, et al.  2015 Jan http://link.springer.com/article/10.1007/s11105-014-0848-4
Post-translational Modifications of Baculovirus Protamine-like Protein P6.9 and the Significance of Its Hyperphosphorylation for Viral Very Late Gene Hyperexpression American Society for Microbiology   Ao Li, et al. 2015 May http://jvi.asm.org/content/early/2015/05/11/JVI.00333-15.abstract
Ribonuclease J is required for chloroplast and embryo
development in Arabidopsis
Journal of Experimental Botany 5.526 Hongyu Chen et al. 2014 Dec http://link.springer.com/article/10.1007/s11105-014-0790-5#

 

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