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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRPCR SuperMix / 2×TransTaq High Fidelity(HiFi)PCR SuperMix I(-dye)
  • Product Name: 2×TransTaq High Fidelity(HiFi)PCR SuperMix I(-dye)
  • Catalog Number: AS131
  • Date: 2015-11-05
  • Views: 17
  • Sold amount:0Piece
    • Specification: 1 ml(-dye) 5×1 ml(-dye)
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Overview

 

Description

TransTaq® High Fidelity (HiFi) PCR SuperMix I or II is a ready-to-use mixture of TransTaq® High Fidelity (HiFi) DNA polymerase, dNTPs and optimized buffer. TransTaq® High Fidelity (HiFi) PCR SuperMix I is optimized for the amplification of genomic DNA and PCR SuperMix II is optimized for the amplification of λDNA, cDNA or plasmid DNA. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.

TransTaq®High Fidelity (HiFi) PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.

 

Applications

• Complex templates
• GC/AT-rich templates
• Long PCR
• High yield PCR

 

 

Contents& storage

 

 

Citations & references
Literature Journal IF Author Date Link
A novel DNA vaccine expressing the Ag85A-HA2 fusion protein provides protection against influenza A virus and Staphylococcus aureus Virology Journal 2.092 Dai J. et al. 2013 Jan http://www.ncbi.nlm.nih.gov/pubmed/23369570
High prevalence and molecular analysis of macrolide-nonsusceptible Moraxella catarrhalis isolated from nasopharynx of healthy children in China Microbial Drug Resistance 2.364 Liu Y, et al. 2012 Aug http://www.ncbi.nlm.nih.gov/pubmed/22394083
Molecular Adjuvant Ag85A Enhances Protection against Influenza A Virus in Mice Following DNA Vaccination Viruses 2.509 Dai J. et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/23223215 
Detection of astrovirus infection in pigeons (Columbia livia) during an outbreak of diarrhoea Avian Pathology 1.729 Zhao W, et al. 2011 Aug http://www.ncbi.nlm.nih.gov/pubmed/21812714
Microsatellite markers for the Chinese herbaceous peony Paeonia lactiflora (Paeoniaceae) American Journal of Botany 2.586 Li Lin, et al. 2011 Feb http://www.amjbot.org/content/98/2/e16.full.pdf+html
rFliC prolongs allograft survival in association with the activation of recipient Tregs in a TLR5-dependent manner Cellular & Molecular Immunology 4.112 Jing Hao, et al.  2014 Nov http://www.nature.com/cmi/journal/v11/n2/abs/cmi201344a.html
Proteomic analysis on zoxamide-induced sensitivity changes in Phytophthora cactorum Pesticide Biochemistry and Physiology 2.014 Xinyue Mei, et al. 2015 Jan http://www.sciencedirect.com/science/article/pii/S0048357515000243
ORF75 of megalocytivirus RBIV-C1: A global transcription regulator and an effective vaccine candidate Fish & Shellfish Immunology 2.674 Jian Zhang, et al.  2015 May http://www.sciencedirect.com/science/article/pii/S1050464815002235
Molecular cloning and characterization of an Hsp70 gene from the bloom green alga Chaetomorpha valida (Cladophorales, Chlorophyta) Journal of Applied Phycology 2.559 Yunyan Deng, et al. 2014 Apr http://link.springer.com/article/10.1007/s10811-014-0313-7
Parallel tagged amplicon sequencing of relatively long PCR products using the Illumina HiSeq platform and transcriptome assembly Molecular Ecology Resources 3.712 Yan-Jie Feng, et al.  2015 May http://onlinelibrary.wiley.com/doi/10.1111/1755-0998.12429/abstract;jsessionid=
BCBE04B943E618FC02BEE82F84F8C94D.f04t04?deniedAccessCustomised
Message=&userIsAuthenticated=false
Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidumssp. dicoccoides ) in relation to Glu -3evolution Development Genes and Evolution 2.441 Lumin Qin, et al.  2014 Nov http://link.springer.com/article/10.1007/s00427-014-0484-x
Epitope analysis, expression and protection of SAG5A vaccine against Toxoplasma gondii Acta Tropica 2.27 Gang Lu, et al. 2015 Jun http://www.sciencedirect.com/science/article/pii/S0001706X15000613
Near Full-Length Identification of a Novel HIV-1 CRF01_AE/B/C Recombinant in Northern Myanmar AIDS Research and Human Retroviruses 2.325 Zhou Yan-Heng, et al. 2015 May http://online.liebertpub.com/doi/abs/10.1089/AID.2015.0021
Phytoplasma associated with Chinese tallow tree yellowing disease in China represents a new 16SrIII subgroup Forest Pathology 1.373 Y. Gao, et al.    2014 Jun http://onlinelibrary.wiley.com/doi/10.1111/efp.12128/full
Molecular characterizationofanovelhypovirusfromtheplant
pathogenicfungus Fusarium graminearum
Virology 3.321 Pengfei Li, et al. 2015 Jul http://www.sciencedirect.com/science/article/pii/S0042682215001129
Cloning and characterisation of NADPH-dependent cytochrome P450 reductase gene in the cotton bollworm, Helicoverpa armigera Pest Management Science 2.594 Chunqing Zhao, et al. 2014 Jan http://onlinelibrary.wiley.com/doi/10.1002/ps.3538/full

 

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