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Your present location:HomeProductsPCR, RT-PCR, qPCR, qRT-PCRRT-PCR / TransScript Reverse Transcriptase
  • Product Name: TransScript Reverse Transcriptase
  • Catalog Number: AT101
  • Date: 2015-11-05
  • Views: 11
  • Sold amount:0Piece
    • Specification: 10000 units 5×10000 units
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  

 

Overview

 

Description

TransScript® Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.

 

Applications

• First-strand cDNA synthesis
• Multiple copy and low copy gene detection

 

Unit Definition

One unit of TransScript® RT incorporates 1 nmol of deoxyribonucleotide into acid-precipitable material in 10 minutes at 37℃ using Poly(A)/Oligo(dT) as template/primer.

 

5×TS RT Buffer

250 mM KCl, 15 mM MgCl2, 100 mM Tris-HCl pH 8.4

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation FASEB Journal 5.704 Zheng J, et al. 2013 Oct http://www.ncbi.nlm.nih.gov/pubmed/23771927
Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli PLoS One 3.23 Sun D, et al. 2013 http://www.ncbi.nlm.nih.gov/pubmed/23533598
Oxidized Low-Density Lipoprotein Induces Inflammatory Responses in Cultured Human Mast Cells Via Toll-Like Receptor 4 Cellular Physiology and Biochemistry 3.415 Meng Z, et al.  2013 http://www.ncbi.nlm.nih.gov/pubmed/23816956
Curcumin inhibits LPS-induced inflammation in rat vascular smooth muscle cells in vitro via ROS-relative TLR4-MAPK/NF-κB pathways Acta Pharmacologica Sinica 2.354 Meng Z, et al. 2013 Jul http://www.ncbi.nlm.nih.gov/pubmed/23645013
 Ferritin is the key to dietary iron absorption and tissue iron detoxification in Drosophila melanogaster FASEB Journal 5.704 Tang X, Zhou B. 2013 Jan http://www.ncbi.nlm.nih.gov/pubmed/23064556
Side-effects of resveratrol in HepG2 cells: Reduced pten and increased bcl-xl mRNA expression Molecular Medicine Reports 1.17 Zheng M, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22971900
Upregulation of sFlt-1 by trophoblasts induces the barrier dysfunction of glomerular endothelial cells Journal of Huazhong University of Science and Technology 0.385 Zhao J. et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/22173504
Dye-free microRNA quantification by using pyrosequencing with a sequence-tagged stem-loop RT primer Chembiochem 3.944 Jing H. et al. 2011 Apr  http://www.ncbi.nlm.nih.gov/pubmed/21425233
Is endothelial dysfunction of cerebral small vessel responsible for white matter lesions after chronic cerebral hypoperfusion in rats? Journal of the Neurological Sciences 2.353 Huang Y, et al. 2010 Dec  http://www.ncbi.nlm.nih.gov/pubmed/20850139
Effect of manganese exposure on intracellular Ca2+ homeostasis and expression of NMDA receptor subunits in primary cultured neurons Neurotoxicology 3.096 Xu B, et al. 2009 Nov http://www.ncbi.nlm.nih.gov/pubmed/19643132
mRNA Expression of IGF-1 and IGF-1R in Patients with Colorectal Adenocarcinoma and Type 2 Diabetes Archives of Medical Research 2.079 Rui Liu, et al. 2014 May http://www.sciencedirect.com/science/article/pii/S0188440914000526
Regulation of FLOWERING LOCUS T by a MicroRNA in Brachypodiumdistachyon  Plant Cell 9.25 Liang Wu, et al. 2013 Dec http://www.plantcell.org/content/25/11/4363.short
Block and Boost DNA Transfer: Opposite Roles of OmpA in Natural and Artificial Transformation of Escherichia coli PLoS ONE 3.23 Dongchang Sun, et al. 2013 Mar http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0059019
Effects on Liver Hydrogen Peroxide Metabolism Induced by Dietary Selenium Deficiency or Excess in Chickens Biological Trace Element Research 1.307 Jing-Xiu Xu, et al. 2014 Jun http://link.springer.com/article/10.1007/s12011-014-0002-z

 

 

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