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  • Product Name: TransScript First-Strand cDNA Synthesis SuperMix
  • Catalog Number: AT301
  • Date: 2015-11-05
  • Views: 14
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    • Specification: 50 rxns×20 µl Systems 100 rxns×20 µlSystems
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Overview

 

Description

TransScript® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript® RT/RI Enzyme Mix and 2×TS Reaction Mix.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.

 

Highlights

N

 

Applications

Multiple copy and low copy gene detection

 

 

Contents& storage

 

 

Citations & references

 

Literature Journal IF Author Date Link
Transcriptome Analysis of the Zebrafish Model of Diamond-Blackfan Anemia from RPS19 Deficiency via p53-Dependent and-Independent Pathways PLoS One 3.23 Jia Q, et al.  2013 Aug  http://www.ncbi.nlm.nih.gov/pubmed/23990987
Comparative Proteomic Analysis of the Hepatic Response to Heat Stress in Muscovy and Pekin Ducks: Insight into Thermal Tolerance Related to Energy Metabolism PLoS One 3.73 Zeng T, et al.  2013 Oct  http://www.ncbi.nlm.nih.gov/pubmed/24116183
Responses of physiological indexes and leaf epicuticular waxes of Brassica napus to Sclerotinia sclerotiorum infection Plant Pathology 2.729 Ni Y, et al. 2013 Apr http://onlinelibrary.wiley.com/doi/10.1111/ppa.12060/abstract?deniedAccessCustomised
Message=&userIsAuthenticated=false
Plant-mediated RNAi of a gap gene-enhanced tobacco tolerance against the Myzus persicae Transgenic Research 2.609 Mao J, Zeng F. 2013 Aug  http://www.ncbi.nlm.nih.gov/pubmed/23949691
Linolelaidic Acid Induces a Stronger Proliferative Effect on Human Umbilical Vein Smooth Muscle Cells Compared to Elaidic Acid Lipids 2.557 Li X P, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23341188
Molecular Cloning and Functional Analysis of the Duck TLR4 Gene Internation Journal of Molecular Sciences 2.464 Zhao W, et al.  2013 Sep  http://www.ncbi.nlm.nih.gov/pubmed/24025421
OsPOP5, A Prolyl Oligopeptidase Family Gene from Rice Confers Abiotic Stress Tolerance in Escherichia coli Internation Journal of Molecular Sciences 2.464 Tan CM, et al. 2013 Oct  http://www.ncbi.nlm.nih.gov/pubmed/24152437
Pepsinogen A and C genes in turbot (Scophthalmus maximus): Characterization and expression in early development Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2.069 Chi L, et al. 2013 May http://www.ncbi.nlm.nih.gov/pubmed/23499940
Improved primary cell culture and subculture of lymphoid organs of the greasyback shrimp Metapenaeus ensis Aquaculture 2.009 Han Q, et al. 2013 Oct  http://www.sciencedirect.com/science/article/pii/S0044848613002986
Cloning and expression analysis of P-glycoprotein gene in Crassostrea ariakensis Aquaculture 2.009 Xu Y Y, et al. 2013 http://www.sciencedirect.com/science/article/pii/S0044848613005139
Dendrimeric bowties featuring hemispheric-selective decoration of ligands for microRNA-based therapy Biomacromolecules 5.371 Gray WD, et al. 2013 Jan  http://www.ncbi.nlm.nih.gov/pubmed/23145944
Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1 Microbiological Research 1.993 Zhang X, et al. 2013 Jun  http://www.ncbi.nlm.nih.gov/pubmed/23276644
The effects of an RGD-PAMAM dendrimer conjugate in 3D spheroid culture on cell proliferation, expression and aggregation Biomaterials 7.604 Jiang LY, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23340194
Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro Acta biochimica et biophysica Sinica 1.807 Xu C, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23419720
Expression analysis of chlorophyllid α binding protein, a secretory, red fluorescence protein in the midgut of silkworm, Bombyx mori Insect Science 1.786 Chen L, et al. 2013 Mar http://www.ncbi.nlm.nih.gov/pubmed/23956194
Relative catalytic efficiency of ldhL- and ldhD-encoded products is crucial for optical purity of lactic acid produced by lactobacillus strains Applied and Environmental Microbiology 3.829 Zheng Z, et al. 2012 May http://www.ncbi.nlm.nih.gov/pubmed/22344644
The expression of LytM is down-regulated by RNAIII in Staphylococcus aureus Journal of Basic Microbiology 1.198 Chunhua M, et al. 2012 Dec http://www.ncbi.nlm.nih.gov/pubmed/22581788
Hypoxia-inducible factor 1-mediated human GATA1 induction promotes erythroid differentiation under hypoxic conditions Journal of Cellular and Molecular Medicine 4.753 Zhang FL, et al. 2012 Aug http://www.ncbi.nlm.nih.gov/pubmed/22050843
Silencing of a novel tumor metastasis suppressor gene LASS2/TMSG1 promotes invasion of prostate cancer cell in vitro through increase of vacuolar ATPase activity Journal of Cellular Biochemistry 3.062 Xu X, et al.  2012 Jul http://www.ncbi.nlm.nih.gov/pubmed/22573553
Feeding-based RNA interference of a gap gene is lethal to the pea aphid, Acyrthosiphon pisum PLoS One 3.23 Mao J, Zeng F. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23144942
Localization, expression change in PRRSV infection and association analysis of the porcine TAP1 gene International Journal of Biological Sciences 3.168 Sun N, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/22211104
Clock-controlled mir-142-3p can target its activator, Bmal1 BMC Molecular Biology 2.857 Tan X, et al.  2012 Sep  http://www.ncbi.nlm.nih.gov/pubmed/22958478
Role of ghrelin on estrogen and progesterone secretion in the adult rat ovary during estrous cycle Systems Biology in Reproductive Medicine 1.847 Fang F, et al. 2012 Apr http://www.ncbi.nlm.nih.gov/pubmed/22142178
In vitro anti-influenza virus and anti-inflammatory activities of theaflavin derivatives Antiviral Research 3.925 Zu M, et al. 2012 Jun http://www.ncbi.nlm.nih.gov/pubmed/22521753
Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses Molecular Biology Reports 2.506 Jiang L, Pan LJ. 2012 Jun http://www.ncbi.nlm.nih.gov/pubmed/22484790
High-density lipoprotein of patients with type 2 diabetes mellitus elevates the capability of promoting migration and invasion of breast cancer cells International Journal of Cancer 6.198 Pan B, et al. 2012 Jul  http://www.ncbi.nlm.nih.gov/pubmed/21805479
MiR-145 regulates epithelial to mesenchymal transition of breast cancer cells by targeting Oct4 PLoS One 3.23 Hu J, et al. 2012 http://www.ncbi.nlm.nih.gov/pubmed/23049906
Assessment of the treatment effect of baicalein on a model of Parkinsonian tremor and elucidation of the mechanism Life Sciences 2.555 Yu X, et al. 2012 Jul  http://www.ncbi.nlm.nih.gov/pubmed/22634324
 Expression of a nematode symbiotic bacterium-derived protease inhibitor protein in tobacco enhanced tolerance against Myzus persicae Plant Cell Reports 2.509 Zhang H, et al. 2012 Nov http://www.ncbi.nlm.nih.gov/pubmed/22833276
Amplification of an MFS transporter encoding gene penT significantly stimulates penicillin production and enhances the sensitivity of Penicillium chrysogenum to phenylacetic acid Journal of Genetics and Genomics 2.076 Yang J. et al.  2012 Nov http://www.ncbi.nlm.nih.gov/pubmed/23177147
Role of ghrelin on testosterone secretion and the mRNA expression of androgen receptors in adult rat testis Systems Biology in Reproductive Medicine 1.847 Wang L, et al. 2011 Jun http://www.ncbi.nlm.nih.gov/pubmed/21204602
 Hypoxic induction of human erythroid-specific δ-aminolevulinate synthase mediated by hypoxia-inducible factor 1 Biochemistry 3.377 Zhang FL, et al. 2011 Feb  http://www.ncbi.nlm.nih.gov/pubmed/21207956
A multiplex RT-PCR for rapid and simultaneous detection of porcine teschovirus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus in clinical specimens Journal of Virological Methods 1.9 Liu S, et al. 2011 Mar http://www.ncbi.nlm.nih.gov/pubmed/21192983
NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis The Plant Jounal 6.16 Zhang B, et al. 2011 Dec http://www.ncbi.nlm.nih.gov/pubmed/21801253
Expression of p53 in the effects of artesunate on induction of apoptosis and inhibition of proliferation in rat primary hepatic stellate cells PLoS One 3.23 Longxi P, et al. 2011 http://www.ncbi.nlm.nih.gov/pubmed/22053192
Genotoxicity evaluation of low doses of avermectin to hemocytes of silkworm (Bombyx mori) and response of gene expression to DNA damage Pesticide Biochemistry and Physiology 2.111 Shen WF, et al 2011 Nov http://www.sciencedirect.com/science/article/pii/S0048357511001350
Characterization of genes expressed in response to cadmium exposure in the earthworm Eisenia fetida using DDRT-PCR Ecotoxicology and Environmental Safety 2.203 Wang X, et al.  2010 Sep http://www.ncbi.nlm.nih.gov/pubmed/20674023
Functional analyses of ethylene response factor JERF3 with the aim of improving tolerance to drought and osmotic stress in transgenic rice Transgenic Research 2.609 Haiwen Zhang, et al. 2010 Oct http://www.ncbi.nlm.nih.gov/pubmed/?term=Functional+analyses+of+ethylene+response+factor+
JERF3+with+the+aim+of+improving+tolerance+to+drought+and+osmotic+stress+in+transgenic+rice
Circulating miR-30a, miR-195 and let-7b associated with acute myocardial infarction PLoS One 3.23 Long G, et al. 2012 Jul http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050926
Selective Protection of Normal Cells During Chemotherapy by RY4 Peptides Molecular Cancer Research 4.353 Xiaorong Wu, et al. 2014 May http://mcr.aacrjournals.org/content/early/2014/05/29/1541-7786.MCR-13-0425.short
Metabolomics Coupled with Multivariate Data and Pathway Analysis on Potential Biomarkers in Gastric Ulcer and Intervention Effects of Corydalis yanhusuo Alkaloid PLoS One 3.23 Li Tianjiao, et al. 2014 Jan http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0082499
Molecular characterization, expression profiling, and binding properties of odorant binding protein genes in the whitebacked planthopper, Sogatella furcifera COMP BIOCHEM PHYS B 1.551 Ming He, et al. 2014 Aug http://www.sciencedirect.com/science/article/pii/S1096495914000475
A new nanoPCR molecular assay for detection of porcine bocavirus Journal of Virological Methods 1.9 Xiaoling Wang, et al. 2014 Jun http://www.sciencedirect.com/science/article/pii/S016609341400086X
A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation The Journal of Biological Chemistry 4.651 Kaiyuan Wu, et al. 2014 Jun http://www.jbc.org/content/early/2014/06/03/jbc.C114.561860.short
The effects of an RGD-PAMAM dendrimer conjugate in 3D spheroid culture on cell proliferation, expression and aggregation Biomaterials 7.604 Jiang LY,et al. 2013 Jan http://www.sciencedirect.com/science/article/pii/S0142961213000112
Gestational Hypoxia Induces Sex-Differential Methylation of Crhr1 Linked to Anxiety-like Behavior Molecular Neurobiology 5.7 Xi Wang,et al.  2013 Mar http://link.springer.com/article/10.1007/s12035-013-8444-4
PDCD5 transfection increases cisplatin sensitivity and decreases invasion in hepatic cancer cells Oncology
Letters
1.554 Gui‑Ling Fan, et al. 2014 Oct http://www.spandidos-publications.com/ol/9/1/411?text=fulltext
Increased Tim-3 expression on peripheral T lymphocyte subsets and association with higher disease activity in systemic lupus erythematosus Diagnostic Pathology  2.597 Li-jun Song, et al. 2015 Jun http://www.diagnosticpathology.org/content/10/1/71/
The effects of gibberellins and mepiquat chloride on nitrogenase activity in Bradyrhizobium japonicum Acta Physiologiae Plantarum 1.584 Wenhao Chen, et al. 2014 Dec http://link.springer.com/article/10.1007/s11738-014-1723-5
Epoxyeicosatrienoic Acids Regulate Macrophage Polarization and Prevent LPS-Induced Cardiac Dysfunction Journal of Cellular Physiology 3.839 Meiyan Dai, et al.  2015 May http://onlinelibrary.wiley.com/doi/10.1002/jcp.24939/abstract?systemMessage=Wiley+Online+Library+
will+be+disrupted+on+11th+July+2015+at+10%3A00-16%3A00+BST+%2F+05%3A00-11%3A00
+EDT+%2F+17%3A00-23%3A00++SGT++for+essential+maintenance.++Apologies+for+the+inconvenience
NE Strengthens the Immunosuppression Induced by AlCl3 Through β2 -AR/cAMP Pathway in Cultured Rat Peritoneal Macrophages Biological Trace Element Research 1.748 Miao Li, et al.   2015 Jan http://link.springer.com/article/10.1007/s12011-014-0217-z
Expression of Brassica napus TTG2, a regulator of trichome development, increases plant sensitivity to salt stress by suppressing the expression of auxin biosynthesis genes Journal of Experimental Botany 5.526 Qingyuan Li, et al. 2015 Jun http://jxb.oxfordjournals.org/content/early/2015/06/11/jxb.erv287.abstract
Heat shock protein 47 is up-regulated in conjunctival bleb scarring after filtration surgery in rats Graefe's Archive for Clinical and Experimental Ophthalmology 1.908 Weiwei Wang,et al. 2015 Jan http://link.springer.com/article/10.1007/s00417-014-2818-9#

 

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