Your present location:HomeProductsCloning and Mutagenesis SystemCloning Vectors / pEASY®-T1 Cloning Kit
  • Product Name: pEASY®-T1 Cloning Kit
  • Catalog Number: CT101
  • Date: 2015-12-21
  • Views: 329
  • Sold amount:0Piece
    • Specification: 20 rxns 60 rxns
  • Quantity:  

Purchase Favorite


Overview Contents& storage Citations & references Related Images Download  


pEASY ®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.
•5 minutes fast ligation of Taq-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.


Contents & storage





(20 rxns)

(60 rxns)


pEASY ®-T1 Cloning Vector (10 ng/μl)


3×20 µl

Control Template (5 ng/μl)



Control Primers (10 μM)



M13 Forward Primer (10 μM)



M13 Reverse Primer (10 μM)



Trans1-T1 Phage Resistant

Chemically Competent Cells

10×100 μl

30×100 μl


Citations & references
Literature Journal IF Author Date Link
Undifferentiated embryonic cell transcription factor-1 (UTF1) inhibits the growth of cervical cancer cells by transactivating p27 Kip1 Carcinogenesis 5.635 Wu X L, Zheng P S. 2013 Jul
Rapid, Simple and Sensitive Detection of Q Fever by Loop-Mediated Isothermal Amplification of the htpAB Gene PLoS Neglected Tropical Diseases 4.569 Pan L, et al. 2013 May
Conversion of upland to paddy field specifically alters the community structure of archaeal ammonia oxidizers in an acid soil Biogeosciences 3.754 Alam M S, et al. 2013
Description of Streptomonospora sediminis sp. nov. and Streptomonospora nanhaiensis sp. nov., and reclassification of Nocardiopsis arabia (Hozzein & Goodfellow, 2008) as Streptomonospora arabica comb. nov. and emended description of the genus Streptomonospora International Journal of Systematic and Evolutionary Microbiology 2.112 Zhang DF, et al. 2013 Jul
Diversity of Wolbachia in Natural Populations of Spider Mites (genus Tetranychus): Evidence for Complex Infection History and Disequilibrium Distribution Microbial Ecology 3.277 Zhang Y K, et al. 2013 Apr
Microbiota associated with the migration and transformation of chlorinated aliphatic hydrocarbons in groundwater Environmental Geochemistry and Health 2.076 Guan X, et al. 2013 Aug
Cloning and haplotype analysis of TaSTE, which is associated with plant height in bread wheat (Triticum aestivum L.). Molecular Breeding 3.251 Zhang W, et al. 2013 Jan
Target-site basis for resistance to acetolactate synthase inhibitor in Water chickweed (Myosoton aquaticum L.) Pesticide Biochemistry and Physiology 2.111 Liu W, et al. 2013 Sep
Bromodichloromethane induces cell proliferation in different tissues of male F344 rats by suppression of E-cadherin expression via hypermethylation or transcriptional activation of c-myc and cyclin D1 Toxicology Letters 3.145 Liao J, et al. 2013 Aug
Bioremediation of Cd and carbendazim co-contaminated soil by Cd-hyperaccumulator Sedum alfredii associated with carbendazim-degrading bacterial strains Environmental Science and Pollution Research International 2.618 Xiao W, et al. 2013 Jan
Substitution mutation induced migration anomaly of a D10S2325 allele on capillary electrophoresis International Journal of Legal Medicine 2.686 Chen PY, et al. 2013 Mar
Molecular Characterization of msp2/p44 of Anaplasma phagocytophilum Isolated from Infected Patients and Haemaphysalis longicornis in Laizhou Bay, Shandong Province, China PLoS One 3.73 Wang Y, et al. 2013 Oct
Deep Sequencing of Maize Small RNAs Reveals a Diverse Set of MicroRNA in Dry and Imbibed Seeds PLoS One 3.73 Li D, et al. 2013
Construction of a bicistronic vector for the co-expression of two genes in Caenorhabditis elegans using a newly identified IRES Biotechniques 2.399 Li D, Wang M. 2012 Mar
Rapid and sensitive detection of Plesiomonas shigelloides by loop-mediated isothermal amplification of the hugA gene PLoS One 3.73 Meng S, et al. 2012
A practicable detection system for genetically modified rice by SERS-barcoded nanosensors Biosensors and Bioelectronics 5.437 Chen K, et al. 2012 Apr
Two mechanisms underlying the loss of p16(Ink4a) function are associated with distinct tumorigenic consequences for WS MEFs escaping from senescence Mechanisms of Aging and Development 3.264 Wu X, et al. 2012 Aug
Isolation and Identification of Cellulolytic Bacteria from the Gut of Holotrichia parallela Larvae (Coleoptera: Scarabaeidae) International Journal of Molecular Sciences 2.598 Huang S, et al. 2012
The TetR-type transcriptional repressor RolR from Corynebacterium glutamicum regulates resorcinol catabolism by binding to a unique operator, rolO Applied and Environmental Microbiology 3.829 Li T, et al. 2012 Sep
Isolation, screening, and optimization of the fermentation conditions of highly cellulolytic bacteria from the hindgut of Holotrichia parallela larvae (Coleoptera: Scarabaeidae) Applied Biochemistry and Biotechnology 1.893 Sheng P, et al. 2012 May
Phylogenetically diverse cultivable fungal community and polyketide synthase (PKS), non-ribosomal peptide synthase (NRPS) genes associated with the South China Sea sponges Microbial Ecology 3.277 Zhou K, et al. 2011 Oct
Rapid, simple, and sensitive detection of Anaplasma phagocytophilum by loop-mediated isothermal amplification of the msp2 gene Journal of Clinical Microbiology 4.068 Pan L, et al. 2011 Dec
Identification of novel maize miRNAs by measuring the precision of precursor processing BMC Plant Biology 4.354 Jiao Y, et al. 2011 Oct
Archaeal and bacterial diversity in hot springs on the Tibetan Plateau, China Extremophiles 2.203 Huang Q, et al. 2011 Sep
Study on Heterotrophic-Autotrophic Denitrification Permeable Reactive Barriers (HAD PRBs) for In Situ Groundwater Remediation          
Is the c. 3G> C mutation in the succinate dehydrogenase subunit D (SDHD) gene due to a founder effect in Chinese head and neck paraganglioma patients? Laryngoscope 1.979 Zha Y, et al. 2011 Aug
Complementary DNA Cloning and Functional Characterization of Cytochrome P450 3A138 in Common Carp (Cyprinus carpio L.) Biochemical and Molecular Toxicology 1.596 Junguo Ma, et al. 2014 Mar


Development of an immunomagnetic bead separation-coupled quantitative PCR method for rapid and sensitive detection of Cryptosporidium parvum oocysts in calf feces Parasitology Research 2.852 Shanshan Gao, et al. 2014 Jun
Cloning and characterization of MxHA7, a plasma membrane H+-ATPase gene related to high tolerance of Malus xiaojinensis to iron deficiency Acta Physiologiae Plantarum 1.305 Qian Zha, et al. 2014 Apr
Neuroprotective Effect of TAT-14-3-3ε Fusion Protein against Cerebral Ischemia/Reperfusion Injury in Rats PLoS One 3.73 Yuanjun Zhu, et al. 2014 Mar
Overexpression of UDP-glucose pyrophosphorylase from Larix gmelinii enhances vegetative growth in transgenic Arabidopsis thaliana Plant Cell Reports 2.509 Ningning Li, et al. 2014 May
Molecular evidence revealed Lepus hainanus and L.peguensis have a conspecific relationship Mitochondrial DNA 1.705 Lingming Kong, et al. 2014 Feb
Hypomethylation of long interspersed nuclear element-1 promoter is associated with poor outcomes for curative resected hepatocellular carcinoma Liver International 3.87 Xu-dong Gao, et al. 2014 Jan
Molecular characterization, expression profile, and polymorphism of goose dopamine D1 receptor gene Molecular Biology Reports 2.506 Cui Wang, et al. 2014 May
Molecular cloning, expression and polymorphism of goose LRP8 gene British Poultry Science 1.147 C. Wang, et al. 2014 Jul


Epigenetic Silencing of RASSF10 Promotes Tumor Growth in Esophageal Squamous Cell Carcinoma Discovery Medicine 2.965 Di Lu, et al. 2014 Apr
Regulation of Tissue LC-PUFA Contents, D6 Fatty Acyl Desaturase (FADS2) Gene Expression and the Methylation of the Putative FADS2 Gene Promoter by Different Dietary Fatty Acid Profiles in Japanese Seabass (Lateolabrax japonicus) PLOS ONE 3.75 Houguo Xu, Xiaojing Dong,et al. 2014 Jan
An Adaptive Transposable Element Insertion in the Regulatory Region of the EO Gene in the Domesticated Silkworm, Bombyx mori Molecular Biology and Evolution 14.308 Wei Sun, et al. 2014 Oct
A maize wall-associated kinase confers quantitative resistance to head smut Nature Genetics 29.648 Weiliang Zuo et al. 2014 Dec
A manganese superoxide dismutase (MnSOD) from ark shell, Scapharca broughtonii: molecular
characterization, expression and immune activity analysis
Fish and Shellfish Immunology 3.034 Libing Zheng, et al. 2014 May
Identification, Expression Profiling and Fluorescence-Based Binding Assays of a Chemosensory Protein Gene from the Western Flower Thrips, Frankliniella occidentalis PLoS One 3.75 Zhi-Ke Zhang, et al. 2015 Jan


In vitro characterization of a (E)-β-farnesene synthase from Matricaria recutita L. and its up-regulation by methyl jasmonate Gene 2.138 Shanshan Su, et al. 2015 Jun
Mutations and amplification of EPSPS gene confer resistance to glyphosate in goosegrass (Eleusine indica ) Planta 3.263 Jingchao Chen, et al. 2015 May
Enhancement of Gellan Production in Sphingomonas paucimobilis JLJ by Heterogeneous Expression of Vitreoscilla Hemoglobin Advances in Applied Biotechnology 1.362 Qinglong Ji, et al. 2015 Jan
Development of 10 microsatellite markers from Pantala flavescens and their applicability in studying genetics diversity Molecular Biology Reports 2.024 Lingzhen Cao, et al. 2015 Mar
pTC Plasmids from Sulfolobus Species in the Geothermal Area of Tengchong, China: Genomic Conservation and Naturally-Occurring Variations as a Result of Transposition by Mobile Genetic Elements Life 9.322 Xiaoyu Xiang, et al. 2015 Feb
Development of a nanoparticle-assisted PCR (nanoPCR) assay for detection of mink enteritis virus (MEV) and genetic characterization of the NS1 gene in four Chinese MEV strains BMC Veterinary Research 1.777 Jianke Wang, et al. 2015 Jan
Down-regulation of a novel ABC transporter gene (Pxwhite) is associated with Cry1Ac resistance in the diamondback moth,Plutella xylostella (L.) Insect Biochemistry and Molecular Biology 3.45 Zhaojiang Guo, et al. 2015 Jan
Identification and functional characterization of the TAB2 gene from Litopenaeus vannamei Fish & Shellfish Immunology 2.674 Sheng Wang, et al. 2015 Jun
Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod -A1 on wheat chromosome 3AL Theoretical and Applied Genetics 3.79 Jingxin Wei, et al. 2015 Jul
Effects of dietary glutamine on survival, growth performance, activities of digestive enzyme, antioxidant status and hypoxia stress resistance of half-smooth tongue sole (Cynoglossus semilaevis Günther) post larvae Aquaculture 1.878 Jingwei Liu, et al. 2015 Apr
A novel NAC transcription factor from Suaeda liaotungensis K. enhanced transgenic Arabidopsis drought, salt, and cold stress
Plant cell rep 3.071 Xiao-lan Li, et al. 2014 Mar
Cloning, identification of the two cytokine receptor family B subunits CRFB1 and CRFB5 from grass carp (Ctenopharyngodon idella) Fish & Shellfish Immunology 2.674 Huarong Chen, et al. 2015 Apr
The novel ABC transporter ABCH1 is a potential target for RNAi-based insect pest control and resistance management Scientific Reports 5.578 Zhaojiang Guo, et al. 2015 Sep
Shifts in Abundance and Diversity of Soil Ammonia-Oxidizing Bacteria and Archaea Associated with Land Restoration in a Semi- Arid Ecosystem PLOS ONE 3.234 Zhu Chen, et al. 2015 Jun
A novel protease inhibitor in Bombyx mori is involved in defense against Beauveria bassiana Insect Biochemistry and Molecular Biology 3.246 Youshan Li, et al. 2012 Jul
Related Images

  To be closed automatically in X seconds

View my favorites