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  • Product Name: pEASY®-T3 Cloning Kit
  • Catalog Number: CT301
  • Date: 2015-12-22
  • Views: 200
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    • Specification: 20 rxns 60 rxns
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pEASY ®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.

•5 minutes fast ligation of Taq-amplified PCR products.

•Ampicillin resistance genes for selection.

•Easy blue/white selection.

•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.

•T7 promoter and SP6 promoter for in vitro transcription.

•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.



Contents& storage





(20 rxns)

(60 rxns)


pEASY ®-T3 Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans1-T1 Phage Resistant

Chemically Competent Cell

10×100 μl

30×100 μl


Citations & references
Literature Journal IF Author Date Link
Evolution and Expression Plasticity of Opsin Genes in a Fig Pollinator, Ceratosolen solmsi PLoS One 3.73 Wang B. et al. 2013
The impact of dissolved organic carbon on the spatial variability of methanogenic archaea communities in natural wetland ecosystems across China Applied Microbiology and Biotechnology 3.689 Liu D, et al. 2012 Oct
Arsenic induces functional re-expression of estrogen receptor α by demethylation of DNA in estrogen receptor-negative human breast cancer PLoS One 3.73 Du J, et al. 2012
Complex evolution in Arundinarieae (Poaceae: Bambusoideae): incongruence between plastid and nuclear GBSSI gene phylogenies Molecular Phylogenetics and Evolution 4.066 Zhang YX, et al. 2012 Jun
DsbM, a novel disulfide oxidoreductase affects aminoglycoside resistance in Pseudomonas aeruginosa by OxyR-regulated response Journal of Microbiology 1.276 Wang X, et al. 2012 Dec
Sinomyces: a new genus of anamorphic Pleosporaceae Fungal Biology 2.082 Wang Y, et al. 2011 Feb
Identification of ethylene-responsive genes in ethrel-treated shoot apices of cucumber by suppression subtractive hybridization Plant Molecular Biology Reporter 5.319 Jin X, et al. 2011 Dec

Three novel species of Stemphylium from Sinkiang, China: their morphological and molecular characterization Mycological Progress 1.606 Pei YF, et al. 2011 May
Molecular and morphological description of two new species of Stemphylium from China and France Mycologia 2.11 Wang Y, et al. 2010 May-Jun
Ulocladium cantlous sp. nov. isolated from northwestern China: its morphology and molecular phylogenetic position Mycologia 2.11 Wang Y, et al. 2010 Mar-Apr
Molecular characterization, immunohistochemical localization and expression of a ribosomal protein L17 gene from Apis cerana cerana Insect Biochemistry and Physiology 1.515 Fei Meng, et al. 2010 Oct
The complete mitochondrial genome of Spilonota lechriaspis Meyrick (Lepidoptera: Tortricidae) Molecular Biology Reports 2.506 Jin-Liang Zhao, et al. 2011 Aug
Halomonas songnenensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils IJSEM   Juquan Jiang,et al. 2014 May
Characterization of a mitochondrial manganese superoxide dismutase gene from Apis cerana cerana and its role in oxidative stress Journal of Insect Physiology 2.379 Haihong Jia, et al. 2014 Jan
Cryptic diversity in host-associated populations of Tetra pinnatifidae (Acari: Eriophyoidea): What do morphometric, mitochondrial and nuclear data reveal and conceal? Bulletin of Entomological Research 1.987 Hao-Sen Li, et al. 2014 Apr


Identification of Rhizoctonia solani AG1-IB as a causal agent of leaf blight in Cynanchum paniculatum in China Journal of General Plant Pathology 0.893 Ai Xin Wang, et al. 2014 Jan
MOLECULAR CHARACTERIZATION OF SOLUBLE AND MEMBRANE-BOUND TREHALASES IN THE COTTON MIRID BUG, Apolygus lucorum Archives of Insect Biochemistry and Physiology 1.515 Yongan Tan, et al. 2014 Jun


RNAi mediated knockdown of the ryanodine receptor gene decreases chlorantraniliprole susceptibility in Sogatella furcifera Pesticide Biochemistry and Physiology 2.111 Yao Yang, et al. 2014 Jan
Pollen and seed transmission of Cucumber green mottle mosaic virus in cucumber Plant Pathology 2.729 H.W.Liu, et al. 2014 Feb

Biochemical functions of the glutathione transferase supergene family of Larix kaempferi Plant Physiology and Biochemistry 2.775 Qi Yang, et al. 2014 Apr
RNA interference-mediated silencing of a Halloween gene spookier affects nymph performance in the small brown planthopper Laodelphax striatellus Insect Science 1.786 Shuang Jia, et al. 2014 Apr;jsessionid=CCB2CD4081BEAA2C0



Differential Expression Patterns in Chemosensory and Non-Chemosensory Tissues of Putative Chemosensory
Genes Identified by Transcriptome Analysis of Insect Pest the Purple Stem Borer Sesamia inferens (Walker)
PLOS one 3.7 Zhang, YN,et al. 2013 Aug
A novel bifunctional pectinase from Penicilliumoxalicum SX6with separate pectin methylesterase and polygalacturonasecatalytic domains Appl Microbiol Biotechnol 3.689 Tao Tu, et al. 2014 Jan
Two Origins of Blastemal Progenitors Define Blastemal
Regeneration of Zebrafish Lower Jaw
PloSOne 3.54 Xuelong Wang, et al. 2014 Dec
Two Metallothionein Genes in Oxya chinensis: Molecular
Characteristics, Expression Patterns and Roles in Heavy
Metal Stress
PloSOne 3.54 Yaoming Liu, et al. 2014 Nov
High Level Expression of a Novel Family 3 Neutral β-Xylosidase from Humicola insolens Y1 with High Tolerance to D-Xylose PloSOne 3.54 Wei Xia, et al. 2015 Feb
Electrical signals generated by soil microorganisms in microbial fuel cells respond linearly to soil Cd 2+ pollution Geoderma 2.772 Yun-Bin Jiang, et al. 2015 Apr Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, School of Geography Science, Nanjing Normal University, 210023 Nanjing, China
Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of R-Smads to Ensure TGFβ-mediated Germ Layer Induction in Xenopus Embryos The Journal of Biological Chemistry 4.573 Guanni Sun, et al. 2015 May
Biochemical characterization of a novel thermophilic α-galactosidase from Talaromyces leycettanus JCM12802 with significant transglycosylation activity Journal of Bioscience and Bioengineering 1.884 Caihong Wang, et al. 2015 Jun
Post-translational Modifications of Baculovirus Protamine-like Protein P6.9 and the Significance of Its Hyperphosphorylation for Viral Very Late Gene Hyperexpression Journal of Virology 4.439 Ao Li, et al. 2015 May
High-yield production of a low-temperature-active polygalacturonase for papaya juice clarification Food chemistry 3.334 Tao Tu,et al. 2013 Jun
Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice Genome Biology 10.465 Yu-Chan Zhang, et al. 2014 Dec
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching. Nature Biotechnology 39.08 Yu-Chan Zhang, et al. 2013 Sep
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