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  • Product Name: pEASY®-T5 Zero Cloning Kit
  • Catalog Number: CT501
  • Date: 2015-12-22
  • Views: 362
  • Sold amount:0Piece
    • Specification: 20 rxns 60 rxns
  • Quantity:  

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pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.

•5 minutes fast ligation of Taq-amplified PCR products.

•High cloning efficiency. Positive clones up to 100%.

•No blue/white selection needed.

•Suitable for short and large fragment cloning.

•Kanamycin and Ampicillin resistance genes for selection.

•M13 forward primer and M13 reverse primer for sequencing.

•T3 promoter and T7 promoter for in vitro transcription.

•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.



Contents& storage





(20 rxns)

(60 rxns)


pEASY ®- T5 Zero Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans 1-T1 Phage Resistant

Chemically Competent Cell

10×100 μl

30×100 μl


Citations & references
Literature Journal IF Author Date Link
High-throughput sequencing reveals the disruption of methylation of imprinted gene in induced pluripotent stem cells Cell Research 10.526 Gang Chang,et al. 2013 Dec
Enhanced Telomere Rejuvenation in Pluripotent
Cells Reprogrammed via Nuclear Transfer
Relative to Induced Pluripotent Stem Cells
Cell Stem Cell 25.315 Rongrong Le et al. 2014 Jan
Identification and RNA Interference of the Pheromone Biosynthesis Activating Neuropeptide (PBAN) in the Common Cutworm Moth Spodoptera litura (Lepidoptera: Noctuidae) Journal of Economic Entomology 1.506 Qin Lu, et al. 2015 Apr
Genome organization and transcriptional regulation of Adenosine Deaminase Acting on RNA gene 1 (ADAR1) in grass carp (Ctenopharyngodon idella) Developmental & Comparative Immunology 2.815 Zhicheng Sun, et al. 2015 Feb
Radiation-Induced Epigenetic Bystander Effects Demonstrated in Arabidopsis Thaliana REGULAR ARTICLES   Wei Xu, et al. 2015 May
The Combination of Tet1 with Oct4 Generates High-Quality Mouse-Induced Pluripotent Stem Cells Embryonic Stem Cells/Induced Pluripotent Stem Cells   Jiayu Chen, et al. 2015 Feb;jsessionid=



Pike , a rice blast resistance allele consisting of two adjacent NBS–LRR genes, was identified as a novel allele at the Pik locus Molecular Breeding 2.246 Jing Chen, et al. 2015 Apr

Identification and Genome Characterization of the First Sicinivirus Isolate from Chickens in Mainland China by Using Viral Metagenomics Plos one 3.234 Hongzhuan Zhou, et al. 2015 Oct
Replacement of Oct4 by Tet1 during iPSC Induction Reveals an Important Role of DNA Methylation and Hydroxymethylation in Reprogramming Cell Stem Cell 25.315 Yawei Gao, et al. 2013 Mar
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