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Your present location:HomeProductsCloning and Mutagenesis SystemCloning Vectors / pEASY®-Blunt3 Cloning Kit
  • Product Name: pEASY®-Blunt3 Cloning Kit
  • Catalog Number: CB301
  • Date: 2015-12-23
  • Views: 36
  • Sold amount:0Piece
    • Specification: 20 rxns 60 rxns
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

pEASY ®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Pfu-amplified PCR products.
•Ampicillin resistance gene for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

 

 

Contents & storage

 

Component

CB301-01

CB301-02

(20 rxns)

(60 rxns)

 

pEASY®-Blunt3 Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans1-T1 Phage Resistant

Chemically Competent Cell

10×100 μl

30×100 μl

 

Citations & references

N

 

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