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Your present location:HomeProductsCloning and Mutagenesis SystemCloning Vectors / pEASY®-Blunt Zero Cloning Kit
  • Product Name: pEASY®-Blunt Zero Cloning Kit
  • Catalog Number: CB501
  • Date: 2015-12-23
  • Views: 115
  • Sold amount:0Piece
    • Specification: 20 rxns 60 rxns
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  
Overview

Description

pEASY ®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required.
•5 minutes fast ligation of Pfu-amplified PCR products.
•High cloning efficiency. Positive clones up to 100%.
•No blue/white selection needed.
•Suitable for larger fragment cloning.
•Kanamycin and Ampicillin resistance genes for selection.
•M13 forward primer and M13 reverse primer for sequencing.
•T3 promoter and T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.

 

 

Contents& storage

 

Component

CB501-01

CB501-02

(20 rxns)

(60 rxns)

 

pEASY ®-Blunt Zero Cloning Vector (10 ng/μl)

20 µl

3×20 µl

Control Template (5 ng/μl)

5 µl

5 µl

Control Primers (10 μM)

5 µl

5 µl

M13 Forward Primer (10 μM)

50 µl

150 µl

M13 Reverse Primer (10 μM)

50 µl

150 µl

Trans 1-T1 Phage Resistant

Chemically Competent Cells

10×100 μl

30×100 μl

 

Citations & references
Literature Journal IF Author Date Link
Molecular cloning and characterization of the mitogen-activated protein kinase kinase gene (MKK4) and its promoter sequence from oilseed rape (Brassica campestris L.). Plant Cell, Tissue and Organ Culture (PCTOC) 3.633 Zhang T G, et al. 2013 Aug http://link.springer.com/article/10.1007/s11240-013-0366-3
Expression, purification and renaturation of truncated human integrin β1 from inclusion bodies of Escherichia coli Protein Expression and Purification 1.695 Tonglin Shi, et al. 2014 Nov http://www.sciencedirect.com/science/article/pii/S104659281400268X
The Different Potential of Sponge Bacterial Symbionts in N2 Release Indicated by the Phylogenetic Diversity and Abundance Analyses of Denitrification Genes, nirK and nosZ PLoS One 3.73 Zhang X, et al. 2013 Jun http://www.ncbi.nlm.nih.gov/pubmed/23762300
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