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Your present location:HomeProductsCloning and Mutagenesis SystemCloning Vectors / pEASY®-Uni Seamless Cloning and Assembly Kit
  • Product Name: pEASY®-Uni Seamless Cloning and Assembly Kit
  • Catalog Number: CU101
  • Date: 2015-12-23
  • Views: 74
  • Sold amount:0Piece
    • Specification: 25 ml
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Overview Contents& storage Citations & references Related Images Download  
Overview
Description
This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.
 
•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.

 

Contents & storage

 

 

Component

CU101-01

CU101-02 CU101-03

2×Assembly Mix

50 μl

100 μl 3×100 μl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl

 10×100 μl  30×100 μl

Linearized pUC19 Control Vector (10 ng/μl)

3 μl

6 μl 18 μl

Control Insert (1 kb,20 ng/μl)

3 μl

6 μl 18 μl

 

Citations & references

 

Literature Journal IF Author Date Link
Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway Metabolic Engineering 8.201 Wang Y, et al. 2017 Jan http://www.ncbi.nlm.nih.gov/pubmed/27815192
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system new phytologist 7.672 Borong Lin, et al.           2015 Oct http://www.ncbi.nlm.nih.gov/pubmed/?term=A+novel+nematode+effector+suppresses+plant+immunity+by+activating+host+reactive+oxygen+species-scavenging+system
The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles. Plant journal 5.468 Li S, et al. 2015 Oct http://www.ncbi.nlm.nih.gov/pubmed/26303437

 

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