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  • Product Name: pEASY®-Uni Seamless Cloning and Assembly Kit
  • Catalog Number: CU101
  • Date: 2015-12-23
  • Views: 136
  • Sold amount:0Piece
    • Specification: 25 ml
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Overview Contents& storage Citations & references Related Images Download  
This kit takes advantage of proprietary assembly mix and homologous recombination. This kit can achieve directional cloning of PCR fragments that share 15-25 bp overlapping sequences into any linearized vector.
•Fast: 15 minutes.
•Broad: no restriction enzyme digestions. Can be cloned into any sites.
•High efficiency: up to 95% cloning efficiency.
•Seamless: no extra sequences introduced; up to 5 fragments assembly.


Contents & storage





CU101-02 CU101-03

2×Assembly Mix

50 μl

100 μl 3×100 μl

Trans1-T1 Phage Resistant Chemically Competent Cell

5×100 μl

 10×100 μl  30×100 μl

Linearized pUC19 Control Vector (10 ng/μl)

3 μl

6 μl 18 μl

Control Insert (1 kb,20 ng/μl)

3 μl

6 μl 18 μl


Citations & references


Literature Journal IF Author Date Link
Switch of metabolic status: redirecting metabolic flux for acetoin production from glycerol by activating a silent glycerol catabolism pathway Metabolic Engineering 8.201 Wang Y, et al. 2017 Jan
A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system new phytologist 7.672 Borong Lin, et al.           2015 Oct
The maize transcription factor EREB58 mediates the jasmonate-induced production of sesquiterpene volatiles. Plant journal 5.468 Li S, et al. 2015 Oct


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