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Your present location:HomeProductsCloning and Mutagenesis SystemCloning Competent Cells / Trans5α Chemically Competent Cell
  • Product Name: Trans5α Chemically Competent Cell
  • Catalog Number: CD201
  • Date: 2015-12-25
  • Views: 24
  • Sold amount:0Piece
    • Specification: 10×100 µl 20×100 µl
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Overview

Description

Trans5α Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 108 cfu/μg DNA (tested by pUC19 plasmid DNA).

 

Genotype

F-φ80d lacZΔM15 Δ(lacZYA-argF) U169 end A1 recA1 hsdR17 (rk-, mk+) supE44λ- thi-1 gyrA96 relA1 phoA

 

Features

• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).

• Reduced recombination of cloned DNA.

• Blue/white selection.

 

Procedures

•Equilibrate a water bath to 42oC.

•Warm a vial of SOC medium or LB medium to room temperature. Warm selective plates at 37oC for 30 minutes.

•Thaw a vial of 100 μl of Trans10 Chemically Competent Cell on ice, aliquot 50 μl of the cells into a prechilled 1.5 ml tube, add target DNA (1 to 5 μl ) into the tube. Do not mix by pipetting up and down. Incubate the cells on ice for 30 minutes.

•Heat-shock the cells for 30 seconds at 42oC without shaking. Immediately transfer the tube to ice. Incubate on ice for 2 minutes without shaking.

•Add 500 μl of prewarmed SOC medium or LB medium (without antibiotic) into the tube, mix well and shake at 37oC for 1 hour at 200 rpm for cell recovery and for the expression of antibiotic resistance.

•Spread 20 to 200 μl from each transformation vial on a prewarmed selective plate. The remaining can be stored at 4oC and plated the next day if needed.

•Invert the plate and incubate at 37oC overnight.

•Select colonies and analyze by restriction enzyme digestion, PCR, or sequencing.

 

Notes

•Higher efficiency transformation can be achieved by transforming cells immediately following thawing.

•Avoid repeated thawing.

•Gentle handling is required for the entire procedure.

 

Contents& storage

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Citations & references
Literature Journal IF Author Date Link
Penicillin-binding protein 3 of Streptococcus pneumoniae and its application in screening of β-lactams in milk Analytical Biochemistry 2.582 Zhang J, et al. 2013 Nov http://www.ncbi.nlm.nih.gov/pubmed/23928045
cDNA cloning and sequence determination of the pheromone biosynthesis activating neuropeptide from the seabuckthorn carpenterworm, Holcocerus hippophaecolus (Lepidoptera:Cossidae). Archives of Insect Biochemistry and Physiology 1.515 Li J, et al. 2013 Apr http://www.ncbi.nlm.nih.gov/pubmed/23361644
Rapid visual detection of phytase gene in genetically modified maize using loop-mediated isothermal amplification method Food Chemistry 3.334 Xin Huang, et al. 2014 Aug http://www.sciencedirect.com/science/article/pii/S0308814614001344
Characterization of a novel carbonic anhydrase from freshwater pearl mussel Hyriopsis cumingii and the expression profile of its transcript in response to environmental conditions Gene 2.196 Gang Ren, et al. 2014 Aug http://www.sciencedirect.com/science/article/pii/S0378111914006027
Cortactin expression confers a more malignant phenotype to gastric cancer SGC-7901 cells World Journal of Gastroenterology 2.547 Jun Wei, et al. 2014 Mar http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964399/
MTERF1 regulates the oxidative phosphorylation activity and cell proliferation in HeLa cells Acta Biochimica et Biophysica Sinica 1.807 Guiyuan Chen, et al. 2014 Apr

http://www.ncbi.nlm.nih.gov/pubmed/21519913

http://abbs.oxfordjournals.org/content/early/2014/04/25/abbs.gmu029.short Plant Breeding 1.598 Huiying Li, et al. 2014 Apr

http://onlinelibrary.wiley.com/doi/10.1111/pbr.12140/abstract;jsessionid=7F774C9CC172CAAB7D17D52D77ADBDCF.f02t04?deniedAccessCustomisedMessage=&userIsAuthenticated=false

Genetic Diversity of Salp15 in the Ixodes ricinus Complex (Acari: Ixodidae) PLoS One 3.73 Xin Wang, et al. 2014 Apr

http://www.plosone.org/article/authors/info%3Adoi%2F10.1371%2Fjournal.pone.0094131

Cloning, expression promoter analysis of vasa gene in Japanese flounder (Paralichthys olivaceus) comparative biochemistry and physiology b-biochemistry & molecular biology 1.551 Xiaomeng Wu, et al. 2014 Jan http://www.sciencedirect.com/science/article/pii/S1096495913000997
Modulation of cell wall synthesis and susceptibility to vancomycin by the two-component system AirSR in Staphylococcus aureus NCTC8325 BMC Microbiology 3.104 Haipeng Sun,et al. 2013 Oct http://www.biomedcentral.com/1471-2180/13/286/
Quantitative monitoring of 2-oxoglutarate in Escherichia coli cells by a fluorescence resonance energy transfer-based biosensor Appl Microbiol Biotechnol 3.689 Chang Zhang, et al. 2013 Jul http://link.springer.com/article/10.1007/s00253-013-5121-5
Enzymological Characterization of Atm, the First Laccase from Agrobacterium sp. S5-1, with the Ability to Enhance In Vitro digestibility of Maize Straw PLoS One 3.53 W Si, et al. 2015 May

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0128204

Cloning of a caffeoyl-coenzyme A O-methyltransferase from Camellia sinensis and analysis of its catalytic activity Journal of Zhejiang University SCIENCE B 1.278 Yue Zhang, et al. 2015 Mar http://link.springer.com/article/10.1631/jzus.B1400193
Cloning and Bioinformatics Analysis of spsC Gene from Sphingomonas sanxanigenens NX02 Advances in Applied Biotechnology   Xiaoyan Li, et al. 2015 Jan http://link.springer.com/chapter/10.1007/978-3-662-45657-6_1
Engineered Expression Vectors Significantly Enhanced the Production of 2-Keto-d-gluconic Acid by Gluconobacter oxidans Journal of Agricultural and Food Chemistry 2.912 Yuan-yuan Shi, et al. 2015 May

http://pubs.acs.org/doi/abs/10.1021/acs.jafc.5b01652

Genomic organization and promoter analysis of a transcriptional repressor gene from Fenneropenaeus chinensis Molecular Biology Reports 2.024 Xiaofang Lai, et al. 2015 Feb

http://link.springer.com/article/10.1007/s11033-014-3780-7

Role of two amino acid residues’ insertion on thermal stability of thermophilic α-amylase AMY121 from a deep sea bacterium Bacillus sp. SCSIO 15121 Bioprocess and Biosystems Engineering 1.997 Lizhen Li, et al. 2014 Nov http://link.springer.com/article/10.1007/s00449-014-1330-2
Hepatitis B virus promotes autophagic degradation but not replication in autophagosome BioScience Trends 1.663 Huayu Yang, et al. 1905/7/7 https://www.jstage.jst.go.jp/article/bst/9/2/9_2015.01049/_article
Construction of a plasmid for overexpression of human circadian gene period2 and its biological activity in osteosarcoma cells Tumor Biology 3.611 An-yuan Cheng, et al. 2015 May http://link.springer.com/article/10.1007/s13277-014-3013-7
Structure-guided creation of AcAP5-derived and platelet targeted factor Xa inhibitors Biochemical Pharmacology 5.009 Yuanjun Zhu, et al. 2015 Apr http://www.sciencedirect.com/science/article/pii/S0006295215001975
Development and characterization of polymorphic microsatellite loci for Saxifraga egregia (Saxifragaceae) Appl Plant Sci. 0.667 Faqi Zhang, et al. 2015 Aug http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4542942/
Automatic Enumeration of Gold Nanomaterials at the Single-Particle Level Anal. Chem. 5.636 Xiao Xu, et al. 2015 Feb http://pubs.acs.org/doi/abs/10.1021/ac503756f
The Complete Mitochondrial Genome of the Booklouse, Liposcelis decolor: Insights into Gene Arrangement and Genome Organization within the Genus Liposcelis PLoS One 3.53 Shi-Chun Chen, et al. 2014 Mar http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0091902
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