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  • Product Name: Fast Mutagenesis System
  • Catalog Number: FM111
  • Date: 2016-01-04
  • Views: 197
  • Sold amount:0Piece
    • Specification: 10 rxns 20 rxns
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• Primers anneal to the DNA template, mutant strands are synthesized with 2×TransStart® FastPfu PCR SuperMix.

• In vitro digestion of non-mutated parental plasmid (methylated plasmid) with DMT enzyme and in vivo degradation of non-mutated parental plasmid (methylated plasmid) with DMT Chemically Competent Cell, so as to efficiently select mutant clones.



• Mutation sites on both primers to improve mutation efficiency.

• Partially overlapping primers for exponential DNA amplification.

• Fast (4 kb/min) and high fidelity ( 54-fold fidelity as compared to EasyTaq® DNA Polymerase) 2xTransStart® FastPfu PCR SuperMix for DNA amplification.

• Double digestions (in vitro and in vivo) of parental plasmids to enhance mutation efficiency.



Contents& storage



FM111-01 (10 rxns)

FM111-02 (20 rxns)

2×TransStart® FastPfu PCR SuperMix

250 µl


DMT Enzyme (10 units/μl)

10 µl


DMT Chemically Competent Cell

10×50 μl

20×50 μl


1 ml

1 ml

SControl Plasmid (5 ng/µl)

10 µl


SControl Primers (10 µM )

10 µl



Citations & references
Literature Journal IF Author Date Link
Identification of BZR1-interacting proteins as potential components of the brassinosteroid signaling pathway in Arabidopsis through tandem affinity purification Molecular and Cellular Proteomics 7.251 Wang C, et al. 2013 Sep
Mutation in AQP5, Encoding Aquaporin 5, Causes Palmoplantar Keratoderma Bothnia Type The Journal of Investigative Dermatology 6.193 Cao X, et al. 2013 Jul
miR-200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin-2 Carcinogenesis 5.635 Zhang HF, et al. 2013 Sep
Gestational Hypoxia Induces Sex-Differential Methylation of Crhr1 Linked to Anxiety-like Behavior Molecular Neurobiology 5.471 Wang X, et al. 2013 Mar
Chronic Inhibition of Cyclic Guanosine Monophosphate-Specific Phosphodiesterase 5 Suppresses Endoplasmic Reticulum Stress in Heart Failure British Journal of Pharmacology 5.067 Gong W, et al. 2013 Aug
Neuroglobin, a Novel Intracellular Hexa-Coordinated Globin, Functions as a Tumor Suppressor in Hepatocellular Carcinoma via Raf/MAPK/Erk Molecular Pharmacology 4.411 Zhang J, et al. 2013 May
The regulation of the UCH-L1 gene by transcription factor NF-κB in podocytes Cellular Signalling 4.304 Zhang H, et al. 2013 Jul
Distinct post-transcriptional regulation of Igfbp1 gene by hypoxia in lowland mouse and Qinghai-Tibet plateau root vole Microtus oeconomus Molecular and Cellular Endocrinology 4.039 Zhang S, et al. 2013 Aug
Stability and iron oxidation properties of a novel homopolymeric plant ferritin from adzuki bean seeds: A comparative analysis with recombinant soybean seed H-1 chain ferritin Biochimica et Biophysica Acta 3.848 Li M, et al. 2013 Apr
Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalyzing the synthesis of ethylene in Agaricus bisporus Biochimica et Biophysica Acta 3.848 Meng D, et al. 2013 Sep
FoxM1 Promotes Glioma Cells Progression by Up-Regulating Anxa1 Expression PLoS One 3.73 Cheng SX, et al. 2013 Aug
Functional consequences of a novel point mutation in the CYP21A2 gene identified in a Chinese Han patient with nonclassic 21-hydroxylase deficiency Clinical Endocrinology 3.396 Chu X, et al. 2013 Aug
GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress Plant Molecular Biology 3.518 Ni Z, et al. 2013 May
FoxQ1 Promotes Glioma Cells Proliferation and Migration by Regulating NRXN3 Expression PLoS One 3.73 Sun HT, et al. 2013
AtPRK2 Promotes ROP1 Activation via RopGEFs in the Control of Polarized Pollen Tube Growth Molecular Plant 6.126 Chang F, et al. 2013 Jul
Novel POMGnT1 mutations cause muscle-eye-brain disease in Chinese patients Molecular Genetics and Genomics 2.881 Jiao H, et al. 2013 Aug
WNT5A/JNK Signaling Regulates Pancreatic Cancer Cells Migration by Phosphorylating Paxillin Pancreatology 2.043 Wei W, et al. 2013 Jul-Aug
Exome sequencing reveals mutations in TRPV3 as a cause of Olmsted syndrome American Journal of Human Genetics 11.202 Lin Z, et al. 2012 Mar
Enhancing production of bio-isoprene using hybrid MVA pathway and isoprene synthase in E. coli PLoS One 3.73 Yang J, et al. 2012
Let-7b inhibits human cancer phenotype by targeting cytochrome P450 epoxygenase 2J2 PLoS One 3.73 Chen F, et al. 2012
Direct observation of the uptake of outer membrane proteins by the periplasmic chaperone Skp PLoS One 3.73 Lyu ZX, et al. 2012
Maternal xNorrin, a canonical Wnt signaling agonist and TGF-β antagonist, controls early neuroectoderm specification in Xenopus PloS Biology 12.69 Xu S, et al. 2012
Hypoxia-induced up-regulation of aquaporin-1 protein in prostate cancer cells in a p38-dependent manner Cellular Physiology and Biochemistry 3.415 Tie L, et al. 2012
Identification and characterization of a highly conserved crenarchaeal protein lysine methyltransferase with broad substrate specificity Journal of Bacteriology 3.194 Chu Y, et al. 2012 Dec
Structural and catalytic role of two conserved tyrosines in Delta-class glutathione S-transferase from Locusta migratoria Archives of Insect Biochemistry and Physiology 1.515 Zhang X, et al. 2012 Jul
Functional analysis and molecular docking identify two active short-chain prenyltransferases in the green peach aphid, Myzus persicae Archives of Insect Biochemistry and Physiology 1.515 Zhang YL, Li ZX. 2012 Oct
In silico and in vitro analyses identified three amino acid residues critical to the catalysis of two aphid farnesyl diphosphate synthase The Protein Journal 1.126 Sun XF, Li ZX. 2012 Jun
Two novel positive cis-regulatory elements involved in green tissue-specific promoter activity in rice (Oryza sativa L ssp.) Plant Cell Reports 2.509 Ye R, et al. 2012 Jun
PDlim2 selectively interacts with the PDZ binding motif of highly pathogenic avian H5N1 influenza A virus NS1 PLoS One 3.73 Yu J, et al. 2011
A conserved cysteine motif is critical for rice ceramide kinase activity and function PLoS One 3.73 Bi FC, et al. 2011 Mar


LTD is a protein required for sorting light-harvesting chlorophyll-binding proteins to the chloroplast SRP pathway Nature Communications 10.015 Ouyang M, 2011
Prevalence of common mutations in the CYP17A1 gene in Chinese Han population Clinica Chimica Acta 2.85 Bao X, et al. 2011 Jun
A novel low-temperature-active β-glucosidase from symbiotic Serratia sp. TN49 reveals four essential positions for substrate accommodation Applied Microbiology and Biotechnology 3.689 Zhou J, et al. 2011 Oct
A new α-galactosidase from symbiotic Flavobacterium sp. TN17 reveals four residues essential for α-galactosidase activity of gastrointestinal bacteria Applied Microbiology and Biotechnology 3.689 Zhou J, et al. 2010 Dec
A nonsense mutation in the Xeroderma pigmentosum complementation group F (XPF) gene is associated with gastric carcinogenesis Gene 2.196 Zhong-Hua Wei, et al. 2014 Mar
miR-200b suppresses invasiveness and modulates the cytoskeletal and adhesive machinery in esophageal squamous cell carcinoma cells via targeting Kindlin-2 Carcinogenesis Integrative Cancer Research   Hai-Feng Zhang, et al. 2013 Sep
Prediction of the key binding site of odorant-binding protein of Holotrichia oblita Faldermann (Coleoptera: Scarabaeida) Insect Molecular Biology 3.044 X.Zhuang, et al. 2014 Jun;jsessionid=



Functional consequences of a novel point mutation in the CYP21A2 gene identified in a Chinese Han patient with nonclassic 21-hydroxylase deficiency Clinical Endocrinology 3.396 Xiaojing Chu, et al. 2014 Jun
Construction, characterization and expression of full length cDNA clone of sheep YAP1 gene Molecular Biology Reports 2.506 Wei Sun, et al. 2014 Feb
Structural analysis of Mycobacterium tuberculosis ATP-binding cassette transporter subunit UgpB reveals specificity for glycerophosphocholine The FEBS Journal 4.25 Dunquan Jiang, et al. 2014 Jan
Glutathione peroxidase 7 utilizes hydrogen peroxide generated by Ero1α to promote oxidative protein folding. Antioxidants & Redox Signaling 7.2 Wang, Lei,et al. 2014 Feb


Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history. Nature genetics 29.648 Xuming Zhou, et al. 2014 Dec
GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress Plant Molecular Biology 4.15 Zhiyong Ni, et al. 2013 Mar
Identification of a novel selective agonist of PPARc with no promotion of adipogenesis and less inhibition of osteoblastogenesis Scientific Reports 5.078 Chang Liu, et al. 2015 Apr
Endostatin has ATPase activity, which mediated its antiangiogenic and antitumor activities Molecular cancer therapeutics 6.1 Shan Wang, et al. 2015 Mar

Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling Cellular Signalling 4.471 Yang Peng et al. 2014 May
Comparison of droplet digital PCR and conventional quantitative PCR for measuring EGFR gene mutation Experimental and Therapeutic Medicine 1.269 Bo Zhang, et al. 2015 Jan
Crystal structure of the death effector domains of caspase-8 Biochemical and Biophysical Research Communications 2.297 Chen Shen, et al. 2015 May
Structural insights into the substrate specificity of two esterases from the thermophilic Rhizomucor miehei The Journal of Lipid Research 4.421 Shaoqing Yang, et al. 2015 Jun
Identification of a novel partial agonist of liver X receptor α (LXRα) via screening Biochemical Pharmacology 4.65 Ni Li, et al. 2014 Dec
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