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Your present location:HomeProductsCloning and Mutagenesis SystemMutagenesis System / Fast MultiSite Mutagenesis System
  • Product Name: Fast MultiSite Mutagenesis System
  • Catalog Number: FM201
  • Date: 2016-01-04
  • Views: 22
  • Sold amount:0Piece
    • Specification: 10 rxns
  • Quantity:  

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Overview Contents& storage Citations & references Related Images Download  
Overview
Description
Fast MultiSite Mutagenesis System is used for generating mutated PCR fragments by introducing mutation sites on overlapping regions. High fidelity TransStart® FastPfu PCR SuperMix is included for amplification. This kit uses proprietary assembly mix and homologous recombination to seamlessly assemble up to six mutagenesis fragments.
 
• Fast: Amplified with fast & high-fidelity 2×TransStart® FastPfu PCR SuperMix; only 15 minutes for recombination.
• Flexible: Able to be cloned into any site to realize single-site/ multi-site, continuous/non-continuous mutagenesis.
• Efficient: >90% mutagenesis efficiency.
 

Notes

We suggest performing 25 cycles for PCR. For low yield PCR products, we suggest using up to 30 cycles.

 

Contents& storage

 

Kit Contents
Component

FM201-01 (10 rxns)

2×TransStart® FastPfu PCR SuperMix

1 ml

DMT Enzyme (10 units/μl)

30 μl

2×Assembly Mix

50 μl

DMT Chemically Competent Cell

10×50 μl

ddH2O

1 ml

 

Citations & references
Literature Journal IF Author Date Link
Activation of GSNOR transcription by NF-κB negatively regulates NGF-induced PC12 differentiation Informa healthcare   Kaiyuan Wu, et al. 2014 Mar http://informahealthcare.com/doi/abs/10.3109/10715762.2014.906743
A peptide panel investigation reveals the acceptor specificity of O-GlcNAc transferase The FASEB Journal 5.704 Xiaoyan Liu, et al. 2014 Apr http://www.fasebj.org/content/early/2014/04/23/fj.13-246850.abstract
Tyrosine 402 Phosphorylation of Pyk2 Is Involved in Ionomycin-Induced Neurotransmitter Release PLoS ONE 3.73 Zhao Zhang, et al. 2014 Apr http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0094574
Lipid raft-associated β-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin Journal of Leukocyte Biolog 4.289 Tingshuang Xu, et al. 2015 Feb http://www.jleukbio.org/content/97/2/297.short
Evolution of a chimeric aspartate kinase for L-lysine production using a synthetic RNA device Applied Microbiology and Biotechnology 3.337 Junming Wang, et al. 2015 May http://link.springer.com/article/10.1007/s00253-015-6615-0
Crystal structure of the polo-box domain of polo-like kinase 2 Biochemical and Biophysical Research Communications 2.297 Hong-Mei Shan, et al. 2014 Dec http://www.sciencedirect.com/science/article/pii/S0006291X14022098
Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling Cellular Signalling 4.304 Peng Yang, et al. 2014 Sep http://www.sciencedirect.com/science/article/pii/S0898656814001223

 

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