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  • Product Name: DNase I (Rnase Free)
  • Catalog Number: GD201
  • Date: 2016-01-05
  • Views: 24
  • Sold amount:0Piece
    • Specification: 1500 units
  • Quantity:  

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Overview

Description

Deoxyribonuclease I (DNase I) is an endonuclease that degrades doubleand single-strand DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Ribonuclease has been reduced to non-detectable levels. Its activity depends on Mg2+ or Mn2+ ion. DNase I with Mg2+ cuts randomly double strand DNA at any site, DNase I with Mn2+ cuts double strand DNA at the same site to form sticky-end with 1-2 nucleotide or form blunt-end.
Unit Definition One unit is the amount of enzyme required to completely degrade 1 μg pBR322 plasmid DNA in 10 minutes at 37°C.
Source purified from bovine pancreas

 

Contents& storage

Molecular Weight:

32 kDa (monomer)

Activity Test Condition:
40 mM Tris-HCl (pH8.0), 10 mM MgSO4, 1 mM CaCl2, 1 μg of pBR322 DNA
 
Purity:
Free of other DNA endonucleases and exonucleases, free of RNase
 
Storage Buffer:
50 mM Tris-acetate (pH 7.5), 10 mM CaCl2, 50% (v/v)glycerol
 
10×Reaction Buffer:
100 mM Tris-HCl (pH7.5 at 25 °C), 100 mM MgCl2, 1 mM CaCl2

 

Components
 
DNase I (3 units/μl) 1500 units
10×DNase I Reaction Buffer 2×1 ml
200 mM EDTA 1 ml
 

 

 

 

Citations & references

 

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