Proteinase K is a nonspecific serine protease that will hydrolyze a variety of peptide bands. Proteinase K is active in a broad range of temperature and buffers. It cannot be inactivated by metal ions, chelating agents (e.g., EDTA), or detergents such as SDS.
• Active in a wide range of buffers and pH value.
• Incubation temperature: 55-65oC; optimal temperature: 58oC
• Incubation time: 15 minutes to 48 hours; optimal incubation time: 2 hours