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  • Product Name: FlyCut® BamHI
  • Catalog Number: JB101
  • Date: 2016-06-20
  • Views: 102
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Overview

Description

FlyCut® BamHI is expressed and purified from E.coli that carries the recombinant BamHI gene. The molecular weight is 27.5 kDa, with the recognition site at G^GATCC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site

5’...G↓GATCC...3’

3’...CCTAG↑G...5’

Applications

Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

 

Contents& storage

 

 

Citations & references
Literature Journal IF Author Date link
Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence
Oncology 
Letters
1.554 Jian‑Zhi Gao, et al.  . 2016 Jan
http://www.ncbi.nlm.nih.gov/pubmed/?term=Long+non-coding+RNA+HOTAIR+is+a+marker+for+hepatocellular+carcinoma+progression+and+tumor+recurrence

 

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