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  • Product Name: FlyCut® BamHI
  • Catalog Number: JB101
  • Date: 2016-06-20
  • Views: 158
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FlyCut® BamHI is expressed and purified from E.coli that carries the recombinant BamHI gene. The molecular weight is 27.5 kDa, with the recognition site at G^GATCC. The reaction is conducted for 5-10 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm or mammalian CpG methylation.

Recognition Site




Genomic DNA, plasmid DNA, PCR product 

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol 

10×FlyCut® Buffer 

500 mM Tris-Ac pH7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA


Contents& storage



Citations & references
Literature Journal IF Author Date link
Long non-coding RNA HOTAIR is a marker for hepatocellular carcinoma progression and tumor recurrence
1.554 Jian‑Zhi Gao, et al.  . 2016 Jan


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